Protein Info for Shewana3_4004 in Shewanella sp. ANA-3

Annotation: flavocytochrome c (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 582 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF04205: FMN_bind" amino acids 36 to 107 (72 residues), 50 bits, see alignment E=9.9e-17 PF00890: FAD_binding_2" amino acids 134 to 564 (431 residues), 257.6 bits, see alignment E=6.5e-80 PF01266: DAO" amino acids 134 to 337 (204 residues), 30.1 bits, see alignment E=9.3e-11 TIGR01813: flavocytochrome c" amino acids 134 to 578 (445 residues), 576.8 bits, see alignment E=1.4e-177 PF12831: FAD_oxidored" amino acids 134 to 313 (180 residues), 30.7 bits, see alignment E=5.5e-11

Best Hits

Swiss-Prot: 92% identical to URDA_SHEON: Urocanate reductase (urdA) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00244, fumarate reductase flavoprotein subunit [EC: 1.3.99.1] (inferred from 100% identity to shn:Shewana3_4004)

MetaCyc: 92% identical to urocanate reductase (Shewanella oneidensis MR-1)
RXN-14236 [EC: 1.3.99.33]

Predicted SEED Role

"Fumarate reductase flavoprotein subunit (EC 1.3.99.1)" in subsystem Succinate dehydrogenase (EC 1.3.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.1

Use Curated BLAST to search for 1.3.99.1 or 1.3.99.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L2F2 at UniProt or InterPro

Protein Sequence (582 amino acids)

>Shewana3_4004 flavocytochrome c (RefSeq) (Shewanella sp. ANA-3)
MHYKQSIIGLAITALIVAAGCQVTHQIVKSQGTAQGKHGEVQVETTFEDGHIVAIDVLKQ
KENKVLAGAVFRDVKQAIIDNNSVDVDGITGATVTSNALKEAVSKSIEAAGVTLAAVAAA
KKTEAVTPTEYTYDVVVIGSGGAGFSAGLEAMAAGRTAVIIEKMPIVGGNSLISGAEMNV
AGSWVQKNMGITDSKELFISDTLKGGDFKGDPEMVKTMADNAVEAAEWLRDYVKVEFYPD
QLFQFGGHSVKRALIPKGHTGAEVISKFSIKAEEVGLPIHTNTKAERLLQDQTGRVVGVE
ATHNGKTITYHAKRGVIVATGGFSSNMEMRKKYNPELDERYGSTGHSGGTGDGIVMAEQI
HAAAKNMGYIQSYPICSPTSGAIALIADARFFGAVLINQKGERFVEELERRDVISQAILG
QPGRYTYVLWNQAIEDVAHTVEMHQGELKEFTKDGLMHKVDTLEEAAKVFNIPEEKLLAT
IKDVNHYAATGKDEAFNHRSGLVDLSKGPYWILKATPSVHHTMGGLVVDTQTRVLDEKGN
IIPGLFAAGEVTGLTHGTNRLGGNAYTDIIVFGRIAGQEAAK