Protein Info for Shewana3_3939 in Shewanella sp. ANA-3

Annotation: DNA-directed RNA polymerase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 signal peptide" amino acids 1 to 38 (38 residues), see Phobius details PF00675: Peptidase_M16" amino acids 77 to 188 (112 residues), 26.1 bits, see alignment E=7.4e-10 PF05193: Peptidase_M16_C" amino acids 216 to 393 (178 residues), 123.2 bits, see alignment E=1.1e-39

Best Hits

KEGG orthology group: K00960, DNA-directed RNA polymerase [EC: 2.7.7.6] (inferred from 100% identity to shn:Shewana3_3939)

Predicted SEED Role

"Insulinase-like:Peptidase M16, C-terminal"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.6

Use Curated BLAST to search for 2.7.7.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L288 at UniProt or InterPro

Protein Sequence (487 amino acids)

>Shewana3_3939 DNA-directed RNA polymerase (RefSeq) (Shewanella sp. ANA-3)
MKLSFVKSSFITSSLQPTKLMVALALGTSLALSGCASTTAPKRVDTGSFAMPSYDKLVLD
NGLTVYLMPQREVPLITLNAVVRAGAVNDTTAGIAQMTAEGLLLGAAGKSKAEIEQQVDF
LGASLGAEADKEGSYLAADFMAKDTDVMLGLFSAALLSPDFDTAEFDKLKQRAIAGLQQD
KESPRAVIGRYFDKLVFGAHPYGNASSGNRESLEQVTVSQLRAFHKSYYQPANTALTVVG
DFDVAAMKAKLTQTFGQWKGSEKLVQPDLNQGLPKLTQAKVLLVDKPDAMETTFVIGGLG
ISRDNPDYVGLTVVNTILGGRFTSWLNDELRVNAGLTYGARSGFSPYTDAGVFTISTFTK
TETTQEAIDLALKTYARLWEKGVDQATLDSAKAYVKGQFPPKFETSGQLAGLLSGMYLYG
FDDKFINEFQAKVDGLTLEETQRLVKTYFPQKDLQFVLIGNASKIAPIAAKYGQVQAVDI
NAVGFGQ