Protein Info for Shewana3_3872 in Shewanella sp. ANA-3

Name: potE
Annotation: putrescine transporter (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 transmembrane" amino acids 9 to 29 (21 residues), see Phobius details amino acids 39 to 59 (21 residues), see Phobius details amino acids 89 to 112 (24 residues), see Phobius details amino acids 118 to 139 (22 residues), see Phobius details amino acids 151 to 171 (21 residues), see Phobius details amino acids 190 to 210 (21 residues), see Phobius details amino acids 223 to 248 (26 residues), see Phobius details amino acids 271 to 293 (23 residues), see Phobius details amino acids 320 to 341 (22 residues), see Phobius details amino acids 350 to 373 (24 residues), see Phobius details amino acids 385 to 403 (19 residues), see Phobius details amino acids 409 to 427 (19 residues), see Phobius details TIGR00905: transporter, basic amino acid/polyamine antiporter (APA) family" amino acids 1 to 430 (430 residues), 443 bits, see alignment E=1.4e-136 TIGR04299: putrescine-ornithine antiporter" amino acids 5 to 430 (426 residues), 754 bits, see alignment E=5.8e-231 PF13520: AA_permease_2" amino acids 8 to 403 (396 residues), 158.4 bits, see alignment E=2.9e-50 PF00324: AA_permease" amino acids 13 to 411 (399 residues), 81.2 bits, see alignment E=7e-27

Best Hits

Swiss-Prot: 74% identical to POTE_ECOLI: Putrescine transporter PotE (potE) from Escherichia coli (strain K12)

KEGG orthology group: K03756, putrescine:ornithine antiporter (inferred from 99% identity to shm:Shewmr7_0277)

MetaCyc: 74% identical to putrescine transporter PotE (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-69; TRANS-RXN0-211

Predicted SEED Role

"Putrescine/proton symporter, putrescine/ornithine antiporter PotE" in subsystem Polyamine Metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L221 at UniProt or InterPro

Protein Sequence (439 amino acids)

>Shewana3_3872 putrescine transporter (RefSeq) (Shewanella sp. ANA-3)
MSKSANKIGVVQLTIITIVNMMGSGIIMLPTQLAQVGTISILSWLVTAAGSTALAYAFAK
CGMFSKKSGGMGGYAEYAFGRSGNFMANYTYAVSLLIANVAIAISAVGYAAVLLEVNLSP
MAICLATIGVLWLATVANFGGARITGRVSSVTVWGIILPVIGVSLIGWFWFDIDLYKGAW
NPHDMPFFKALGGSIAMTLWAFLGLESACANSETVDNPEKNVPIAVMGGTLGAALIYIVS
TNVIAGIVPNADLANSNAPFGLAFAQMFNPVVGKIVMACAIISCTGSLLGWQFTIAQVFK
ASADEGFFPKVFSKVSKADAPIWGMTIIVSIQTLLSLMTISPSLSKQFEALVNLAVVTNI
VPYILSMAALGVMQKQLKVPANKARVANVIAVIGALYSFYALYSSGETAVMLGAIATFFG
WTIYGVISNKTPTAEIKAA