Protein Info for Shewana3_3794 in Shewanella sp. ANA-3

Annotation: type I restriction-modification system, M subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 574 transmembrane" amino acids 368 to 388 (21 residues), see Phobius details TIGR00497: type I restriction-modification system, M subunit" amino acids 9 to 477 (469 residues), 264.1 bits, see alignment E=1.3e-82 PF12161: HsdM_N" amino acids 10 to 145 (136 residues), 113.6 bits, see alignment E=1.6e-36 PF02384: N6_Mtase" amino acids 158 to 464 (307 residues), 359.7 bits, see alignment E=2.2e-111 PF07669: Eco57I" amino acids 273 to 369 (97 residues), 25.4 bits, see alignment E=2.1e-09

Best Hits

KEGG orthology group: K03427, type I restriction enzyme M protein [EC: 2.1.1.72] (inferred from 100% identity to shn:Shewana3_3794)

Predicted SEED Role

"Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L1U3 at UniProt or InterPro

Protein Sequence (574 amino acids)

>Shewana3_3794 type I restriction-modification system, M subunit (RefSeq) (Shewanella sp. ANA-3)
MTQKLTLQQLESFLWEAADILRGNMDASEYKDYIFGMMFLKRLSDAFEEAQEGVVQYYLG
KGKTKEQAEALAQDEDEYDKTFFVPKSARWSALKDLKHDIGATLNKATEAIEEYNSSLEG
VLVTIDFNIKNKLSDKKLRDLLSHFSKYRLRNEDFERPDLLGTAYEYLIKMFADSAGKKG
GEFYTPSEVVSLLVALLKPKAGMRIYDPTSGSGGMLVQTRNYLAAHGENPGNLSLFGQEM
NLNTWAICKMNMFLHGVLSADIRKGDTLREPKHTEGGELMTFDRVIANPPFSLAKWGKDE
CDNDGFGRFPYGTPPKDAGDLAFVQHMIASTNAEGMVGVVMPHGVLFRGSSEKAIRQGIL
EDDLLEAVIGLPSGLFYGTGIPACLLIINKRKAAERKGKVLFINGELEYEEGKNQNKLRP
QDIEKIVTTFNNYQNIKRYAQVVELSEISDNDYNLNIRRYADTAPPAEIFDVRAILHGGV
PVREVESEYIQEEILQGFDVSSVFTLKSEGYYQFRTDISAKERIREFATDATSAIISQLE
RWWDKYHVSLKQLDDEVATAESVMQGYLKELGYE