Protein Info for Shewana3_3709 in Shewanella sp. ANA-3

Annotation: dTDP-4-dehydrorhamnose reductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 3 to 292 (290 residues), 227 bits, E=6.9e-71
• PF01370: Epimerase
  • amino acids 4 to 209 (206 residues), 99.4 bits, E=5.8e-32
• PF16363: GDP_Man_Dehyd
  • amino acids 5 to 147 (143 residues), 47.9 bits, E=3.4e-16
• PF02719: Polysacc_synt_2
  • amino acids 41 to 148 (108 residues), 27.6 bits, E=4.2e-10

Best Hits

Swiss-Prot: 45% identical to MAT2B_XENTR: Methionine adenosyltransferase 2 subunit beta (mat2b) from Xenopus tropicalis

KEGG orthology group: None (inferred from 100% identity to shn:Shewana3_3709)

MetaCyc: 43% identical to Methionine adenosyltransferase 2 subunit beta (Homo sapiens)
Methionine adenosyltransferase. [EC: 2.5.1.6]

Predicted SEED Role

"dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 1.1.1.133)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• S-adenosyl-L-methionine salvage I (4/4 steps found)
• S-adenosyl-L-methionine biosynthesis (1/1 steps found)
• S-adenosyl-L-methionine salvage II (2/3 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (4/7 steps found)
• L-methionine degradation I (to L-homocysteine) (1/3 steps found)
• ethene biosynthesis I (plants) (1/3 steps found)
• 2'-deoxymugineic acid phytosiderophore biosynthesis (1/4 steps found)
• L-methionine salvage cycle III (6/11 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (1/5 steps found)
• L-methionine salvage cycle I (bacteria and plants) (6/12 steps found)
• L-methionine salvage cycle II (plants) (4/11 steps found)
• superpathway of L-methionine salvage and degradation (4/16 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (6/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism
• Polyketide sugar unit biosynthesis
• Selenoamino acid metabolism
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.133, 2.5.1.6

Use Curated BLAST to search for 1.1.1.133 or 2.5.1.6

Sequence Analysis Tools

See A0L1K8 at UniProt or InterPro

Protein Sequence (309 amino acids)

>Shewana3_3709 dTDP-4-dehydrorhamnose reductase (RefSeq) (Shewanella sp. ANA-3)
MANIMVTGATGLLGRAVVKQLTAAGHRVIATGFSRAEAGIHLLDLTQAAEVEAFIAREQP
EVIVHCAAERRPDVSERSPEHALALNLSASQTLAEVAKTHQAWLLYISTDYVFDGTTPPY
AEDAVPNPVNFYGESKLQGETCVLNTDSGFAVLRLPILYGEVTQLSESAVLVLIKLLLDS
RPQRVDHWAIRAPTSTADIANAIAKLIQRQLHAADVSGIYHFSATQTMTKYQMLISLGEL
LGIDTAHLLPEQTPMDSAKRPQDCTLSCGRLAALGIHSELDFKAGLSRALSQSSAALAAV
GCSLRNHYG