Protein Info for Shewana3_3672 in Shewanella sp. ANA-3

Annotation: primosomal protein N' (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 736 PF17764: PriA_3primeBD" amino acids 6 to 108 (103 residues), 91.9 bits, see alignment E=6.4e-30 PF21213: PriA-like_WH" amino acids 123 to 180 (58 residues), 37 bits, see alignment 9.6e-13 PF04851: ResIII" amino acids 207 to 366 (160 residues), 53.1 bits, see alignment E=1.3e-17 PF00270: DEAD" amino acids 211 to 373 (163 residues), 69.9 bits, see alignment E=7.7e-23 TIGR00595: primosomal protein N'" amino acids 228 to 732 (505 residues), 597.5 bits, see alignment E=1e-183 PF18319: Zn_ribbon_PriA" amino acids 452 to 476 (25 residues), 32.2 bits, see alignment (E = 2.9e-11) PF00271: Helicase_C" amino acids 500 to 596 (97 residues), 32.4 bits, see alignment E=3.3e-11 PF18074: PriA_C" amino acids 642 to 732 (91 residues), 64.4 bits, see alignment E=5e-21

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 100% identity to shn:Shewana3_3672)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L1H2 at UniProt or InterPro

Protein Sequence (736 amino acids)

>Shewana3_3672 primosomal protein N' (RefSeq) (Shewanella sp. ANA-3)
MSLFVEVALPVPMRQNFTYRVDTAEQGEESAQQPQIGIRVKVPFGRQQLIGLVTAITDSC
DLAPTQLKSVIEFIDDAPLLPESLYKLTLWAARYYFCSQGQMLTQALPVALRKGLDAAPQ
KIQYWQITELGNNIAPETLKRAPAQKRLLERLQQACLTQEDVISLELNKAALKTLEDKGW
IARHEKLAELNLDWRSQLELDEAPHRLNKEQAVAVTLLTQQQGYHCTLLEGITGSGKTEV
YLAVLEHILKQGKQALILVPEIGLTPQTINRFKRRFKVNVAVLHSGLTDNQRLEAWRQAR
CGQAAIIIGTRSALFTPMAFPGVIILDEEHDSSFKQQEGVGYHARDLAVMRGHLESIPVI
LGSATPSLETLQNALSGRYHHLQLGERAGNAKKVRQGIIDIKNLPLKAGMSAPLINEIRS
HLEAGNQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQSLGEIRCHHCGNQYAIP
RQCHNCGSTMLMGQGIGTEQLAEALQQEFPKYPVVRIDRDTTRLKGSLESHLSAIHKGEY
KILVGTQMLAKGHHFPDVTLVGLLDVDGALFSADFRAPERFGQLYTQVAGRAGRANKPGT
VLLQTHQSDNPILRDLLHRGYGEFARSQLKERQMALLPPAWHMVLVRAEAHSAVDADNFL
NAFAALLPQDKEFEIIGPMPAPLDRKAGKFRRQLMFQAKHRHRLQQEFERIYPLVEQLPE
AKRCRWSLDRDPQDLL