Protein Info for Shewana3_3539 in Shewanella sp. ANA-3

Annotation: alkaline phosphatase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 546 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00245: Alk_phosphatase" amino acids 76 to 534 (459 residues), 428.2 bits, see alignment E=1.9e-132

Best Hits

KEGG orthology group: K01077, alkaline phosphatase [EC: 3.1.3.1] (inferred from 100% identity to shn:Shewana3_3539)

Predicted SEED Role

"Alkaline phosphatase (EC 3.1.3.1)" in subsystem Phosphate metabolism (EC 3.1.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.1

Use Curated BLAST to search for 3.1.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L143 at UniProt or InterPro

Protein Sequence (546 amino acids)

>Shewana3_3539 alkaline phosphatase (RefSeq) (Shewanella sp. ANA-3)
MNKKLLVLAISAMLGLAACGSDGDNGAAGTPGSNGSNGSNGSDGKDWTAVNQWYVDAQAR
VTKADGLSVNNEVGAAKNVILFVGDGMGVSTITAARILEGQLKGKTGEENSLSFETLPYV
GLSKTYNVDGQTPDSAGTMSAMMTGVKTDVGVISQAEGVVRANCASTKDQNLVTSLELAA
MAGMSTGVVTTARLTHATPAATYAHVPERDWEADSNLPAEAVTNGCKDIAAQMLDFNYGN
GLNVMMGGGRRSFIPKTLIDPEGKAGKRNDGRDLTAEWLAKYTNAAYVQDRDGFLNVDVK
STDHLLGLFNSSHMEYDYDRTEAGVTGEPSLAEMTAKSIDILKKNIKGYVLIVEAGRIDH
AHHAGNAARALYDTVALSEAVRVAMEKTSANDTLLMVTADHSHVFTIAGYPTRGNPILGL
VKSNDANGIAEVTNSTDANGLPYTTVGYANGMGYASLATGGDERYNFPVDAGRKDLNFVD
TQSAGFHQEALVPLSDETHAGEDVAIFARGPSSDLVQGTVEQNHIFHVMNRAADLVAKAE
AAMAAK