Protein Info for Shewana3_3469 in Shewanella sp. ANA-3

Annotation: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 4 to 311 (308 residues), 395.4 bits, E=2.3e-122
  • amino acids 546 to 693 (148 residues), 29.7 bits, E=5e-11
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 6 to 753 (748 residues), 1074.8 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 427 to 749 (323 residues), 505 bits, E=9.9e-156

Best Hits

Swiss-Prot: 98% identical to METE_SHEON: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 100% identity to shn:Shewana3_3469)

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• S-adenosyl-L-methionine salvage I (4/4 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• S-adenosyl-L-methionine salvage II (2/3 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• superpathway of seleno-compound metabolism (8/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.14

Sequence Analysis Tools

See A0L0X3 at UniProt or InterPro

Protein Sequence (756 amino acids)

>Shewana3_3469 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase (RefSeq) (Shewanella sp. ANA-3)
MQLNSLGFPRIGRRRELKFALEKYWRGESTQAELHEVAKELRRTHWQWQVAAGIEQVPVG
DFAFYDQVLTLSATLNAIPDRHRGEGAIDLDTLFRVARGRAPTGTDAPASEMTKYFNTNY
HYLVPELKQDQVFSIAYEQFFDEVAEAQALGYQAKPVLLGPVSYLYLAKTVGQDFDKLSL
LPNLLKAYADILARFAAQGVTWVQLEEPILALELTHDWQAAISEAYQALKTAQVKILLTS
YYGSISHHQALVSALPVAGLHLDLVTAPEQLALFANALRSDQILSAGVVNGRNVWAAEVD
LIVERIGSVARDLGARLWIGTSCSLLHSPVDLEVETTLTPALRQQLAFAKQKLLELANVR
QLLQAPESIAAKEIVNTCLARREAKAQAADAKVIARVAALTQADYERVSEFTERQAVQQR
KYRLPLLPTTTIGSFPQTPAIRGLRSRWRKGELSDAQYTEQLQQVTRDTIDRQLKLGIDV
LVHGEAERNDMVEYFGEQLEGVGFTKNGWVQSYGSRCVKPPLIYGDVSRPKAMTVDWAEF
AQSLTDKPVKGMLTGPVTILHWSFAREDISRDTIATQLALAIRDEVVDLQNAGIGIIQID
EPAFREGLPLKQSEWQAYLDWAVNAFKLSAAGVVDETQIHTHMCYSEFNDTIAAIAAMDA
DVITIETSRSRMELLNAFEDFEYPNEIGPGVYDIHSPNTPSVEAMVHLIEKAAQKVPVRQ
LWVNPDCGLKTRTWDEVEPALKNMVDATRELRRRLG