Protein Info for Shewana3_3282 in Shewanella sp. ANA-3
Name: metH
Annotation: B12-dependent methionine synthase (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 66% identical to METH_VIBCH: Methionine synthase (metH) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 64% identity to azc:AZC_4341)MetaCyc: 55% identical to cobalamin-dependent methionine synthase (Homo sapiens)
Methionine synthase. [EC: 2.1.1.13]
Predicted SEED Role
"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)
MetaCyc Pathways
- aspartate superpathway (23/25 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
- folate transformations II (plants) (11/11 steps found)
- folate transformations III (E. coli) (9/9 steps found)
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
- superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
- superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
- superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
- folate transformations I (10/13 steps found)
- L-methionine biosynthesis I (4/5 steps found)
- L-methionine biosynthesis III (3/4 steps found)
- L-methionine salvage from L-homocysteine (1/3 steps found)
- superpathway of L-methionine salvage and degradation (4/16 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.1.1.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0L0D7 at UniProt or InterPro
Protein Sequence (1244 amino acids)
>Shewana3_3282 B12-dependent methionine synthase (RefSeq) (Shewanella sp. ANA-3) MAISLHRASQTLADIRNQLSKRILILDGAMGTMIQGYKLEEEDYRGERFKDWHTDVKGNN DLLVLTQPHIIKQIHIDYLKAGADIIETNTFNATTIAMADYDMQSLSAEINREGARLARE ACDEIEQATGKPRYVAGVLGPTNRTCSISPDVNDPGYRNIHFDELVTAYCESTRALIEGG ADIIMVETIFDTLNAKAALFAIETVFDELFGPNSPARLPVMISGTITDASGRTLTGQTTE AFYNSLRHIKPLSIGLNCALGPKELRPYVEELSRIAECYVSAHPNAGLPNEFGGYDETPE DMASVIQEWAREGMLNIIGGCCGSTPEHIKVIREAVEPFAPRVLPEIPVACRLSGLEPLT IDAQTLFVNVGERTNVTGSAKFLKLIKEGKFEQALDVAREQVESGAQIIDINMDEGMLDG VEVMHKFLNLIASEPDISRVPIMIDSSKWEVIEAGLKCIQGKGIVNSISLKEGEEKFIEQ ATLVKRYGAAAIIMAFDEQGQADTKARKVEICTRAYRVLVDKVGFPPEDIIFDPNIFAIA TGIDEHDNYAVDFIEAIKEIKATLPHAMISGGVSNVSFSFRGNNPVREAIHAVFLYHAIK VGMDMGIVNAGQLAIYDDIDPELKDKVENVVLNLHCPVEDSNNTEQLLEIAEKFRGDGSS SAKKEDLEWRSWPVNQRLAHALVKGITEFIDEDTEAARQLASRPLDVIEGPLMDGMNIVG DLFGSGKMFLPQVVKSARVMKKAVAYLNPFIEQEKVEGQSNGRILMVTVKGDVHDIGKNI VGVVLACNGFEVFDLGVMVSVERILEAVKEHNIDIIGMSGLITPSLDEMVHNVKTFHREG LTIPAIIGGATCSKIHTAVKIAPHYPHGAIYIADASRAVPMVSKLVSNETRQATIDETYA EYEEMRIKRLSQTKRKEIVSLEAARENRCQHDWANYTPFKPNVLGRQVFDDYPLTDLVDR IDWTPFFRAWELHGHYPEILTDKVVGVEAQKLFADGQAMLKKIIDEKWLTAKGVIGLFPA NTVGFDDIELYTDETRTEVEMTTHHLRMQLERVGNDNFCLADFVAPKDSGVADYMGGFAV TAGHGIDEHIARFEANHDDYNAIMLKCLADRLAEAFAERMHERVRKEFWGYAADEQLDNE ALIREKYKGIRPAPGYPACPDHTEKGLLWDLLKPNETIDLNITESYAMFPTAAVSGWYFA HPKSRYFGVTNIGRDQVEDYAKRKGMTVAETEKWLAPVLDYDPE