Protein Info for Shewana3_3272 in Shewanella sp. ANA-3

Annotation: periplasmic binding protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 274 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF01497: Peripla_BP_2" amino acids 28 to 250 (223 residues), 93.6 bits, see alignment E=6.7e-31

Best Hits

Swiss-Prot: 42% identical to BTUF_VIBVU: Vitamin B12-binding protein (btuF) from Vibrio vulnificus (strain CMCP6)

KEGG orthology group: K06858, vitamin B12 transport system substrate-binding protein (inferred from 100% identity to shn:Shewana3_3272)

Predicted SEED Role

"Vitamin B12 ABC transporter, B12-binding component BtuF" in subsystem Coenzyme B12 biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L0C7 at UniProt or InterPro

Protein Sequence (274 amino acids)

>Shewana3_3272 periplasmic binding protein (RefSeq) (Shewanella sp. ANA-3)
MLMRRFIALGLSFALLPAVAMAEPAKRIIALSPHAVEMLYAIGAGESIVAATDYADYPEA
AKKIPSIGGYYGIQIERVLELNPDLIVVWDTGNKAEDINQLKNLGFKLYSSSPKTLEDVA
KEIEELGELTGHTEQASRVAADYRSELLRLRSENAAKSEPKVFYQLWSTPLMTVAKNSWI
QQIIGVCHGKNVFYDAASDYPQVSLENVLLTLPEVILQSEEEGNVKGVDWSQWAEIPAVK
NQHIYQLNADLLHRATPRALLGVKAVCDALDKAR