Protein Info for Shewana3_2896 in Shewanella sp. ANA-3

Annotation: thiamine biosynthesis protein ThiI (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 484 TIGR00342: tRNA sulfurtransferase ThiI" amino acids 4 to 373 (370 residues), 383.7 bits, see alignment E=9.5e-119 PF22025: ThiI_fer" amino acids 8 to 77 (70 residues), 42.8 bits, see alignment E=1.1e-14 PF02926: THUMP" amino acids 87 to 164 (78 residues), 53.1 bits, see alignment E=7.3e-18 PF02568: ThiI" amino acids 178 to 369 (192 residues), 232.8 bits, see alignment E=5.6e-73 TIGR04271: thiazole biosynthesis domain" amino acids 384 to 484 (101 residues), 139.7 bits, see alignment E=3e-45 PF00581: Rhodanese" amino acids 407 to 482 (76 residues), 26.9 bits, see alignment E=1.1e-09

Best Hits

Swiss-Prot: 100% identical to THII_SHESA: tRNA sulfurtransferase (thiI) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K03151, thiamine biosynthesis protein ThiI (inferred from 100% identity to she:Shewmr4_2726)

MetaCyc: 62% identical to tRNA uridine 4-sulfurtransferase (Escherichia coli K-12 substr. MG1655)
tRNA sulfurtransferase. [EC: 2.8.1.4]; 2.8.1.- [EC: 2.8.1.4]

Predicted SEED Role

"tRNA S(4)U 4-thiouridine synthase (former ThiI) / Rhodanese-like domain required for thiamine synthesis" in subsystem Thiamin biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KZA4 at UniProt or InterPro

Protein Sequence (484 amino acids)

>Shewana3_2896 thiamine biosynthesis protein ThiI (RefSeq) (Shewanella sp. ANA-3)
MKFIVKLYPEIMMKSKPVRMRFTKMLETNIRNVLKKVDEDAKVQRQWDRIWVKVPNDKPE
LAQAFGERLACIPGIAHVVQVDEYSFTSVDDIYQQVLPVYRDQIAGKTFCVRVKRTGSHD
FNSIEVERYVGGGLNQFTDAIGVRLKNPEVTVNLEIEGDKLYMVTKRIEGLGGFPMATQE
DVLSLISGGFDSGVSSYQFIKKGARTHYCFFNLGGAQHEIGVKQVAYHLWKTYGESHKVK
FVSVPFEPVVAEILEKIDNGQMGVVLKRMMMRTAARIAERMGIQAIVTGESLGQVSSQTL
TNLNVIDRCTDMLILRPLIAMDKQDIINECRRIGTEDFAKSMPEYCGVISQKPTVKAVLA
KVEAEETKFSEDLIDRIVEQAVAIDIREIAEQMNTRITETETVVAIDTNEVVIDIRAPEE
EENKPLEIEGVEIKRIPFFKLATQFADLDKQKTYLLYCERGVMSKLQALYLIEQGYHNVK
VYRP