Protein Info for Shewana3_2843 in Shewanella sp. ANA-3

Annotation: 2-nitropropane dioxygenase, NPD (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF18328: PfaD_N
  • amino acids 16 to 84 (69 residues), 57.5 bits, E=1.5e-19
• TIGR02814: PfaD family protein
  • amino acids 87 to 524 (438 residues), 708.3 bits, E=1.8e-217
• PF03060: NMO
  • amino acids 99 to 391 (293 residues), 31.5 bits, E=1.9e-11
• PF21607: FabD_helical_ins
  • amino acids 401 to 480 (80 residues), 116.7 bits, E=6.2e-38

Best Hits

KEGG orthology group: None (inferred from 100% identity to shn:Shewana3_2843)

MetaCyc: 87% identical to icosapentaenoate synthase PfaD subunit (Photobacterium profundum SS9)
2.3.1.-

Predicted SEED Role

"Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), inferred for PFA pathway" (EC 1.3.1.9)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (50/53 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• superpathway of fatty acid biosynthesis II (plant) (37/43 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (20/20 steps found)
• biotin biosynthesis I (14/15 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (9/9 steps found)
• 8-amino-7-oxononanoate biosynthesis I (10/11 steps found)
• (5Z)-dodecenoate biosynthesis I (6/6 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (13/16 steps found)
• cis-vaccenate biosynthesis (5/5 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• (5Z)-dodecenoate biosynthesis II (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
• fatty acid elongation -- saturated (4/5 steps found)
• icosapentaenoate biosynthesis IV (bacteria) (1/1 steps found)
• anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (20/205 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.9

Use Curated BLAST to search for 1.3.1.9

Sequence Analysis Tools

See A0KZ51 at UniProt or InterPro

Protein Sequence (548 amino acids)

>Shewana3_2843 2-nitropropane dioxygenase, NPD (RefSeq) (Shewanella sp. ANA-3)
MTNTTLDNKKLSPWPWQVDEAAISFDIDSLGKKLKDLNQACYLVNHAEKGLGIAQSAEVV
GLAEPSNSLHKSNGLHPVSAFAPALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELV
IALGQAGILCSFGAAGLIPSRVEAAIKRIQTALPNGPYAFNLIHSPSEPALERGSVELFL
KHQVRTVEASAFLGLTPQIVYYRAAGLSRDANGEIVIGNKVIAKISRTEVATKFMEPAPV
KMLQQLVNEGLISEEQMLMAQSVPMADDITAEADSGGHTDNRPLVTLLPTILALKDTIQA
KYQYKTPIRVGAGGGIGTPDAALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEM
ADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRYDSIEAIPAEERQKLEEQVF
RASLDVIWAGTVAHFNERDPKQIERALDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDY
QIWAGPALGAFNAWAKGSYLDDYRERNAVDLAKHLMQGAAYQARINLLLSQGVSIPVSLQ
RWKPLQRC