Protein Info for Shewana3_2817 in Shewanella sp. ANA-3

Annotation: PII uridylyl-transferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 861 TIGR01693: protein-P-II uridylyltransferase" amino acids 17 to 852 (836 residues), 958.1 bits, see alignment E=2.4e-292 PF03445: DUF294" amino acids 30 to 107 (78 residues), 21.7 bits, see alignment E=7.1e-08 PF01909: NTP_transf_2" amino acids 52 to 98 (47 residues), 26.9 bits, see alignment 2.4e-09 PF08335: GlnD_UR_UTase" amino acids 162 to 299 (138 residues), 158 bits, see alignment E=7.1e-50 PF27444: GlnD_3rd" amino acids 322 to 413 (92 residues), 76.1 bits, see alignment E=9.8e-25 PF01966: HD" amino acids 469 to 544 (76 residues), 30.2 bits, see alignment E=2.2e-10 PF27446: GlnD_5th" amino acids 600 to 652 (53 residues), 63.9 bits, see alignment 4.8e-21 PF01842: ACT" amino acids 676 to 728 (53 residues), 28.9 bits, see alignment 3.5e-10

Best Hits

Swiss-Prot: 98% identical to GLND_SHEON: Bifunctional uridylyltransferase/uridylyl-removing enzyme (glnD) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00990, [protein-PII] uridylyltransferase [EC: 2.7.7.59] (inferred from 100% identity to shn:Shewana3_2817)

Predicted SEED Role

"[Protein-PII] uridylyltransferase (EC 2.7.7.59)" in subsystem Ammonia assimilation (EC 2.7.7.59)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KZ25 at UniProt or InterPro

Protein Sequence (861 amino acids)

>Shewana3_2817 PII uridylyl-transferase (RefSeq) (Shewanella sp. ANA-3)
MNTALLAKQSLIEQNQTLLARFKAKTPIEELVTQRCQFVDSLVKQAWQQHGLDQYPIALI
AVGGYGRGELHPHSDVDLLFLFEGELSKAAETALSEFIAFLWDASLEIGHSVRTLSDTLN
LGREDITIATNLLEARLLSGPETLFDQLYDAIRQSDFWTSSQFFIAKRDEQTARHAKASA
FDLEPNIKSCPGGLRDIQTIAWVAMRHFGASRLEELVQYEFLEPAELEELLECQHFLWKL
RFALHMAVGRDENRLLFDVQRQVAELMGYEDATQLAVEQMMKRYYRTVRRVMELNEMLLQ
LFKRATLGQTKALEIQAIDNNYQRRGAFIEALRDDLFDSGEEIVRLFVHVAKNSNIKGIY
APTLRSLRRARRAQQQPLQENPQCRQAFMEILKHPRGIAALSLMHKHGVLSAYLPAWRNI
EGQMQFDLFHAYTVDEHTHRLLLNIERFSQPEQKEEFPLGSVLINQLPKKGLLVLGAIFH
DIAKGRGGDHSKLGSSDALAFCKLHGLNDHDGRLVAWLVENHLVMSVTAQRRDISDPDVV
ADFASKVRDAVHLSYLYCLTVADICATNEKTWNNWKGSLLRDLYFSTQRVLARGKEKPVD
IRARVREYQAKAKKELLRRGVKEKELDTLWQRFKADYFLRHQPNQVAWHAEAILKHKQLD
EPLVLVSKHTTRGGTELFVYCQDRPKLFATVMAVLDNKNINVHDANIMTSKDNYALDTFV
ILEQDGAPVSQLSRIQSIRKALEKALASDNPKLPRFRKLSRKMKPFNVPTQVSFLESNRH
GTSMMELIALDTPGLLAKVGDIFYRCNTTLLAAKITTIGERAEDFFILQTNDGLQLNETQ
ENTLKEALISALSAINTESTN