Protein Info for Shewana3_2603 in Shewanella sp. ANA-3

Annotation: anaerobic ribonucleoside triphosphate reductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03477: ATP-cone
  • amino acids 3 to 88 (86 residues), 67.6 bits, E=1.7e-22
• TIGR02487: anaerobic ribonucleoside-triphosphate reductase
  • amino acids 114 to 704 (591 residues), 653.4 bits, E=1.6e-200
• PF13597: NRDD
  • amino acids 115 to 704 (590 residues), 639.5 bits, E=8.7e-196
• PF01228: Gly_radical
  • amino acids 581 to 685 (105 residues), 104.5 bits, E=6.6e-34

Best Hits

Swiss-Prot: 78% identical to NRDD_ECOLI: Anaerobic ribonucleoside-triphosphate reductase (nrdD) from Escherichia coli (strain K12)

KEGG orthology group: K00527, ribonucleoside-triphosphate reductase [EC: 1.17.4.2] (inferred from 80% identity to dze:Dd1591_3748)

MetaCyc: 78% identical to anaerobic ribonucleoside-triphosphate reductase (Escherichia coli K-12 substr. MG1655)
RIBONUCLEOSIDE-TRIP-REDUCT-RXN [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]

Predicted SEED Role

"Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)" in subsystem Ribonucleotide reduction (EC 1.17.4.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (7/7 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.98.6 or 1.17.4.2

Sequence Analysis Tools

See A0KYG1 at UniProt or InterPro

Protein Sequence (705 amino acids)

>Shewana3_2603 anaerobic ribonucleoside triphosphate reductase (RefSeq) (Shewanella sp. ANA-3)
MPVVIKRDGCRTPFDETRIRDAVIAAANSVGIEDADYAATVAACVRERVANLAEVEIHHL
QDAVENLLMEGPYKSLARVYIEYRHDRDICRDATSKLNLEIRGLVEQSNAALLNENANKD
SKVIPTQRDLLAGIVAKHYAKSHILPKDVVAAHELGEIHYHDLDYSPFFPMFNCMLIDLA
GMLTHGFKMGNAEIETPKSISTATAVTAQIIAQVASHIYGGTTINRIDEVLAPFVAKSYD
KHYQVALNWQIKDAKAYATALTEKECHDAFQSLEYEVNTLHTANGQTPFVTFGFGLGTSW
ESRLIQQSMLKVRMTGLGKNRKTAVFPKLVFAIRDGINHKAGDCNYDVKQMALKCASMRM
YPDILNYEQVEKVTGSFKTPMGCRSFLGSYKENGELVHEGRNNLGVVSLNLPRVALEANG
NEAEFYRILDERLLLARRALDTRIERLNGVKARVAPILYMEGACGVRLRADDDIAQIFKN
GRASISLGYIGLHETINALYGTETHVFDDAKLREKAVAIVAHLKAATDAWKAETGYGFSL
YSTPSESLCSRFCKLDARQFGVVAGVTDKGYYTNSFHLDVEKRVNPYDKIDFEQPYPAIA
NGGFICYGEYPNMQHNIEALENVWDYSYSRVPYYGTNTPIDECYDCGFTGEFSCTSKGFT
CPKCGNHEPSRVSVTRRVCGYLGSPDARPFNHGKQEEVKRRIKHL