Protein Info for Shewana3_2313 in Shewanella sp. ANA-3

Annotation: alpha amylase, catalytic region (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 686 signal peptide" amino acids 1 to 44 (44 residues), see Phobius details PF09087: Cyc-maltodext_N" amino acids 58 to 146 (89 residues), 73.9 bits, see alignment E=1.6e-24 PF00128: Alpha-amylase" amino acids 165 to 543 (379 residues), 153.3 bits, see alignment E=1.8e-48 PF10438: Cyc-maltodext_C" amino acids 594 to 682 (89 residues), 73.6 bits, see alignment E=1.6e-24

Best Hits

KEGG orthology group: None (inferred from 100% identity to shn:Shewana3_2313)

Predicted SEED Role

"Neopullulanase (EC 3.2.1.135)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.135)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.135

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KXM3 at UniProt or InterPro

Protein Sequence (686 amino acids)

>Shewana3_2313 alpha amylase, catalytic region (RefSeq) (Shewanella sp. ANA-3)
MIRRSLFLKSHLTPHPIKAHSMIRHSLLFALVLTASSPLYALAQASHSPVSSTASLHLEP
LSWWAGMHNPKLQLMLHSDALPTDAAQLKVEITGQDVLFEGIEQTDNPHYLFINLDLRQA
KPQTFDIRILAGDKQLLQLPYTLATRAEGSRERQGFNNKDVIYLITPDRFANGNPSNDNQ
SDMLEHAAPDNSDGRHGGDIEGIRQHLDYLAKLGVTQLWINPLLENNQAQYSYHGYAMTN
LYRVDPRFGSNQDYQRLVTEAKQHGLGVIKDVVLNHIGSNHWWLKDLPSSDWLNVPQQPN
ATDTQTAPAASSTSLSSTNLGFTSHRRTTVQDPYADMQDKRDFVDGWFVDSMPDLNQRNP
KLATYLIQNSIWWVEYAGLSGIREDTYSYADKAFLTQWTKALMAEYPHFNIVGEEWTANP
ITVSYWQKGKINADGYSSDLPSLMDFPLYEKLLESLNEPEGWDTGFIKLYEMLANDVVYA
TPSQLVLFEGNHDTNRIYSLLKENLPLYQMALTYVLTAKRIPQLFYGTEVLMTSPTEGRH
DGVVRSDFPGGFANNKVNGFSGKGLNAKQQQALAFVRTLLNYRKHSTALQTGDLLHFVPQ
NGIYVQFRCVAKQAAAGNACYQADTDKVMVIYNKNDQAQTLDLSRFSKVLAGNTSANSVL
SGDKLKLNQSLVLPHAGVMLLELNHQ