Protein Info for Shewana3_2066 in Shewanella sp. ANA-3

Updated annotation (from data): L-arabinose isomerase (EC 5.3.1.4)
Rationale: Specifically important for utilizing L-Arabinose. Automated validation from mutant phenotype: the predicted function (ARABISOM-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: L-arabinose isomerase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 PF02610: AraA_N" amino acids 8 to 174 (167 residues), 270.9 bits, see alignment E=5.8e-85 PF24856: AraA_central" amino acids 177 to 324 (148 residues), 207.5 bits, see alignment E=2e-65 PF11762: Arabinose_Iso_C" amino acids 328 to 470 (143 residues), 203.8 bits, see alignment E=1.5e-64

Best Hits

Swiss-Prot: 100% identical to ARAA_SHESA: L-arabinose isomerase (araA) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K01804, L-arabinose isomerase [EC: 5.3.1.4] (inferred from 100% identity to she:Shewmr4_1979)

Predicted SEED Role

"L-arabinose isomerase (EC 5.3.1.4)" in subsystem L-Arabinose utilization (EC 5.3.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.3.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KWX7 at UniProt or InterPro

Protein Sequence (500 amino acids)

>Shewana3_2066 L-arabinose isomerase (EC 5.3.1.4) (Shewanella sp. ANA-3)
MKAFKQKQVWFITGSQDLYGPKVLEQVAKNSEQIVHGFNESSAISIEVVYKPTVKSPREI
HAVCQAANSDENCVGVILWMHTFSPAKMWIAGLNELSKPFMHLHTQFNAELPWSEINMNY
MNTHQSAHGCREFGFIGTRMRKERKVVVGHWQSSDVQAQIDDWCRAAAGWHESQNLRIAR
FGDNMRQVAVTEGDKVAAQIQFGYEVHAYSLGELNEAIADIAEGDVTAQLDRYASEYQVG
NELFGDEYQLDRLRKEAKIELGLTQFLTQGGFGAFTNCFENLTGMTGLPGLATQRLMANG
FGYGGEGDWKTAAMVRIMKVMGQGRAGGTSFMEDYTYNFGATDQVLGAHMLEVCPSIAAA
KPRLEVHRHTIGVRCDVPRLLFTGKAGPAINVSTIDLGNRFRIILNELDTVTPPQDLPNL
PVASALWEPRPNLAVAAAAWIHAGGAHHSAYSQAITTDQIVDFAEMAGAELVIIDADTKI
REFKNELRQNSVYYGLARGL