Protein Info for Shewana3_1985 in Shewanella sp. ANA-3

Annotation: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 513 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details PF02113: Peptidase_S13" amino acids 48 to 479 (432 residues), 381.7 bits, see alignment E=4.6e-118 TIGR00666: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase" amino acids 72 to 467 (396 residues), 346.6 bits, see alignment E=8e-108 PF13354: Beta-lactamase2" amino acids 280 to 464 (185 residues), 28.4 bits, see alignment E=1.1e-10

Best Hits

KEGG orthology group: K07259, D-alanyl-D-alanine carboxypeptidase / D-alanyl-D-alanine-endopeptidase (penicillin-binding protein 4) [EC: 3.4.16.4 3.4.21.-] (inferred from 100% identity to shn:Shewana3_1985)

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4, 3.4.21.-

Use Curated BLAST to search for 3.4.16.4 or 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KWP7 at UniProt or InterPro

Protein Sequence (513 amino acids)

>Shewana3_1985 D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase (RefSeq) (Shewanella sp. ANA-3)
MTYSLNKDKLQASFTRPKIKGLLLGSLVSMLLSTQAHAAIHPMDESILAKQIADIAPRHS
QVALLARDLSTNTLLYSQQADTLFIPASTQKVLTAVTALATLGPDFRYVTELWSDAPIRQ
GHIAGSVYLRFSGDPTLTQDDLKALFAHLQKQGITSIEGHLYLIGDKQEQLQAPGWVWDD
LGICFAAPVSSYIINQNCVYGQFAPSSAKHASEVKLRASSFGVKVSSDAIFSPKAGHDFC
QLDLVRLGQNQYHLRGCYPGNEAIPLAIAISDPEKFAMDTLTATLKGEMSLSGKVKIGNA
IPSKAKLIASHSSAPLPELLKTMLLKSDNLIADSLFKRVGQSYYKAQGSFTHGAAAMRHI
LTELGIDLTNANIVDGSGLSRYNLLSAKQLADVLALIYQDARFHSLIDSLPEAGVSGTLQ
YRLGYTKPPLKHRVFAKTGSMQGVANLAGFMRLAQQRDILFVVLENGISPETKKQRKPAF
SADFLTRLLSAVETQTQTSQTTTTELNTVKLSD