Protein Info for Shewana3_1953 in Shewanella sp. ANA-3

Name: aspS
Annotation: aspartyl-tRNA synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 TIGR00459: aspartate--tRNA ligase" amino acids 1 to 585 (585 residues), 833.3 bits, see alignment E=4.7e-255 PF01336: tRNA_anti-codon" amino acids 18 to 100 (83 residues), 58.4 bits, see alignment E=8.6e-20 PF00152: tRNA-synt_2" amino acids 119 to 557 (439 residues), 411.9 bits, see alignment E=2.2e-127 PF02938: GAD" amino acids 309 to 408 (100 residues), 97.2 bits, see alignment E=8e-32

Best Hits

Swiss-Prot: 100% identical to SYD_SHESA: Aspartate--tRNA ligase (aspS) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 100% identity to shn:Shewana3_1953)

MetaCyc: 69% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KWL5 at UniProt or InterPro

Protein Sequence (591 amino acids)

>Shewana3_1953 aspartyl-tRNA synthetase (RefSeq) (Shewanella sp. ANA-3)
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGLVQVVYDPDLPEVFNVA
STLRAEFCVQVKGVVRARPDSQVNAQMKTGEIEVLGKELTIINSSEPLPLSLDNYQNNSE
EQRLKYRYLDLRRPEMAQRLMFRAKVTSAVRRFLDSNGFLDIETPILTKATPEGARDYLV
PSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MTSEQVMAKTEEMMRGLFLEMLNVDLGEFPRMTYNEAMRRFGSDKPDLRNPLELVDVADL
LKEVEFAVFSGPANDEEGRVAALRIPGGASLSRKQIDDYTKFVGIYGAKGLAWMKLNDLT
QGLEGIQSPVLKFLNEGIVNEIISRTGAQTGDIILFGADNATVVAESMGALRLKAGEDFN
LLEGQWRPLWVVDFPMFEKINGSFHAVHHPFTAPRGVTPQELEANPANRVSDAYDMVLNG
CELGGGSVRIHNQEMQSAVFRILGITDEEAKEKFGFLLEALRYGTPPHAGLAFGLDRIIM
LMTGASSIRDVMAFPKTTTAACPLTNAPGFANPQQLAELGISVVKAAKTED