Protein Info for Shewana3_1601 in Shewanella sp. ANA-3

Name: rlmL
Annotation: 23S rRNA m(2)G2445 methyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 713 PF22020: RlmL_1st" amino acids 3 to 57 (55 residues), 53.5 bits, see alignment 1e-17 PF02926: THUMP" amino acids 67 to 151 (85 residues), 50 bits, see alignment E=1.8e-16 PF01170: UPF0020" amino acids 162 to 371 (210 residues), 179.7 bits, see alignment E=3.2e-56 PF10672: Methyltrans_SAM" amino acids 459 to 668 (210 residues), 85.4 bits, see alignment E=2.2e-27 PF03602: Cons_hypoth95" amino acids 542 to 689 (148 residues), 39.4 bits, see alignment E=2.9e-13 PF13847: Methyltransf_31" amino acids 544 to 669 (126 residues), 28.5 bits, see alignment E=6.8e-10 PF09445: Methyltransf_15" amino acids 547 to 645 (99 residues), 22.2 bits, see alignment E=5.2e-08

Best Hits

Swiss-Prot: 100% identical to RLMKL_SHESA: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K12297, ribosomal RNA large subunit methyltransferase L [EC: 2.1.1.173] (inferred from 100% identity to shn:Shewana3_1601)

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KVL6 at UniProt or InterPro

Protein Sequence (713 amino acids)

>Shewana3_1601 23S rRNA m(2)G2445 methyltransferase (RefSeq) (Shewanella sp. ANA-3)
MLNFFAAAPKGFEYSLAQELTEFGATEIKESVAGVYFTAPLALAYRITLWTRLASRIVLV
IYKGPCESAEQLYNAAYCIDWSAHFSNRNTFSIDFHGTGGFINNTQFGALKIKDAIVDRF
RDDGDARPNVARIDADIKIDAHFRNGVITIAMNFSGPSLHQRGYRSTTGEAPLKENLAAN
MLVRSGWKAAPTTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKATWHEI
LEEAKARASLGVKRCDVKFYGSDIDSRLVALAKRNAQNAGVFELIDFKVANALNVEPPAA
EGYLITNPPYGERLGSVSELLQLYYQLGDKFKKEFGGWKVAMLCSDIELISALKLKADKQ
MKMFNGALECAFNLYTLHAQSTRRDTPVLPEGVDIADIAPAFANRIKKNAKQLEKWAKKE
GIDSYRLYDADIPEYNVAVDRYLDHIVVQEYMAPASIPEAVTKRRLSDVLLALPAAIGVD
PHKITMKTRERQKGTNQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLV
GQKSKGRRVLNLFSYTGSASVHAALGGAKSVTTVDMSNTYLAWAKENFALNDLSGKQYEF
VQADCLQWIRDSAHDKSAQYDLIFIDPPTFSNSKRMEDSFDVQRDHVNLLGMLIKLLSPN
GEIVFSNNKRKFKMDTDTLVKMKIKVENIDDLTLPMDYKRNPHIHNTWLITHA