Protein Info for Shewana3_1530 in Shewanella sp. ANA-3

Annotation: putative periplasmic protease (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 181 to 198 (18 residues), see Phobius details PF08496: Peptidase_S49_N" amino acids 2 to 149 (148 residues), 187 bits, see alignment E=2.2e-59 PF01343: Peptidase_S49" amino acids 152 to 299 (148 residues), 160.5 bits, see alignment E=3.3e-51

Best Hits

Swiss-Prot: 56% identical to SOHB_SHIFL: Probable protease SohB (sohB) from Shigella flexneri

KEGG orthology group: K04774, serine protease SohB [EC: 3.4.21.-] (inferred from 100% identity to shn:Shewana3_1530)

Predicted SEED Role

"Possible protease sohB (EC 3.4.21.-)" (EC 3.4.21.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KVE5 at UniProt or InterPro

Protein Sequence (338 amino acids)

>Shewana3_1530 putative periplasmic protease (RefSeq) (Shewanella sp. ANA-3)
MEFLYEYGMFLAKAVTIVVALVAVIIVVLASTVKHKSDKGELRITNLSEELEELKHSLKE
ELLSKKQFKAYEKQLKADEKAKEKTAEEDSKGKVFVIDFKGSIDAAEVASLREEISAILT
IAEKGDEVVVNVESGGGMVHGYGLASSQLDRLRQAEIPLTICVDKVAASGGYMMACVANK
VYAAPFAIVGSIGVVAQLPNFNRLLKKHEIDYEQHTAGDFKRTLTVFGENTDEGRQKFQQ
ELEETHVLFKAFVSKYRPQLDLAKVATGEHWYGQQAIELGLIDAISTSDDVLMQLAGERT
VYKLRYQVRKKLADKIAHGASLSVNAIFNRLVEKNRPM