Protein Info for Shewana3_1381 in Shewanella sp. ANA-3

Annotation: dTDP-4-dehydrorhamnose reductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 PF04321: RmlD_sub_bind" amino acids 1 to 287 (287 residues), 356.9 bits, see alignment E=2.1e-110 TIGR01214: dTDP-4-dehydrorhamnose reductase" amino acids 2 to 288 (287 residues), 340.2 bits, see alignment E=4e-106 PF01370: Epimerase" amino acids 3 to 175 (173 residues), 84.6 bits, see alignment E=2.3e-27 PF01073: 3Beta_HSD" amino acids 35 to 232 (198 residues), 22.2 bits, see alignment E=2e-08 PF16363: GDP_Man_Dehyd" amino acids 35 to 139 (105 residues), 50.3 bits, see alignment E=7.9e-17 PF02719: Polysacc_synt_2" amino acids 37 to 158 (122 residues), 26.9 bits, see alignment E=8.3e-10

Best Hits

Swiss-Prot: 53% identical to RMLD_AGGAC: dTDP-4-dehydrorhamnose reductase (rmlD) from Aggregatibacter actinomycetemcomitans

KEGG orthology group: K00067, dTDP-4-dehydrorhamnose reductase [EC: 1.1.1.133] (inferred from 100% identity to shn:Shewana3_1381)

MetaCyc: 53% identical to dTDP-6-deoxy-L-talose 4-dehydrogenase (NAD+) (Aggregatibacter actinomycetemcomitans)
RXN-13749 [EC: 1.1.1.339]

Predicted SEED Role

"dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 1.1.1.133)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.133

Use Curated BLAST to search for 1.1.1.133 or 1.1.1.339

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KUZ6 at UniProt or InterPro

Protein Sequence (291 amino acids)

>Shewana3_1381 dTDP-4-dehydrorhamnose reductase (RefSeq) (Shewanella sp. ANA-3)
MKILVTGSNGQVGSCLVKLLNQIPEIEFLAVDREQLDITDYEAVNKLVSEFKPDAIINAA
AHTAVDKAEQEVELSYAINRDGPQFLAQAANSVGATILHISTDYVFAGDKVGEYVETDEV
APQGIYGKSKLAGELAVAAACPRHIIMRTAWVFCETGNNFIKTMLRLAQSRDELGVVADQ
FGGPTYAGDIANTLIEIAQALYAGNTHYGIYHFSGLPHVSWYQFSCQIFKQAKAQKLVSK
DMLINGISSEQYPTPVKRPANSKLSCRKIEQTFGVKPCDWQSALNNLMGYF