Protein Info for Shewana3_1240 in Shewanella sp. ANA-3

Annotation: phosphoribosylformylglycinamidine synthase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1293 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 4 to 1290 (1287 residues), 2041 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 35 to 149 (115 residues), 146.8 bits, see alignment E=6.5e-47 PF18072: FGAR-AT_linker" amino acids 170 to 219 (50 residues), 78.3 bits, see alignment (E = 1.2e-25) PF02769: AIRS_C" amino acids 430 to 586 (157 residues), 120.4 bits, see alignment E=2.1e-38 amino acids 834 to 965 (132 residues), 55.1 bits, see alignment E=2.6e-18 PF22689: FGAR-AT_PurM_N-like" amino acids 646 to 804 (159 residues), 276.3 bits, see alignment E=1.7e-86 PF13507: GATase_5" amino acids 1038 to 1289 (252 residues), 358.7 bits, see alignment E=3.6e-111

Best Hits

Swiss-Prot: 70% identical to PUR4_ALIF1: Phosphoribosylformylglycinamidine synthase (purL) from Aliivibrio fischeri (strain ATCC 700601 / ES114)

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 67% identity to aap:NT05HA_1480)

MetaCyc: 70% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KUK6 at UniProt or InterPro

Protein Sequence (1293 amino acids)

>Shewana3_1240 phosphoribosylformylglycinamidine synthase (RefSeq) (Shewanella sp. ANA-3)
MEIIRGAPALSTFRVQKLMEACVNAALPVRQIYAEYVHLADLSELLETNEREQLEKILTY
GPAIEAHTPQGSLLFVTPRPGTISPWSSKATDIAHNCGLGKVKRLERGVAYYVESDTLTV
EQQQTLKGLLHDRMVEVVLDDFAKADVLFKRTEPAPFKSVNVLAEGRRALEVANVEMGLA
LAEDEIDYLVENFVRLNRNPNDIELMMFAQANSEHCRHKIFNADWTIDGEAQPKSLFKMI
KNTFETTPDHVLSAYKDNAAVMEGSVAGRFFPDPNGVYSYHTEPMHVLMKVETHNHPTAI
SPYPGAATGSGGEIRDEGATGRGSKPKAGLTGFSVSNLKIPGFVQPWEGNYGKPERIVSA
LDIMTEGPLGGAAFNNEFGRPALLGYFRTYEQEVSSHNGVEVRGYHKPIMLAGGLGNIRE
EHVQKGEITVGAKLIVLGGPAMNIGLGGGAASSMASGQSSEDLDFASVQRENPEMERRCQ
EVIDRCWQLGDKNPIQFIHDVGAGGLSNAFPELVNDGGRGGIFNLRNVPSDEPGMSPLEI
WCNESQERYVLSVAAEDLPLFTAICERERAPFAVVGEATQEQHLTLADSHFDNNPIDLPL
EVLLGKAPKMSRNVVSAKAVSPALEQSNIDVKEAVKRILSLPTVADKTFLITIGDRTVTG
LVNRDQMVGPWQVPVADCAVTAASFDTYAGEAMSMGERTPLALLDFGASARMAVAESIMN
IAGADIGSFKRIKLSANWMSAAGHPGEDAGLYEAVKAVGEELCPELSLTIPVGKDSMSMK
TAWQQDGANKTVTAPMSLVISAFGVVQDIRNTVTPELRSDKGETSLLLVDLGAGKNRLGG
SCLAQVYGELGDIAPDLDDAALLRGFFETMQKLVAKKSVIAYHDRSDGGLFTTLVEMAFA
GNTGLAIDLSALQGTDVERLFNEELGGVLQVSRADAELIAAQFAQAGVPCHMIGTLANDQ
RVTIKDGAREVFSETRVALRTLWSETTYRMQALRDNPACALEEFKLKQDETDLGLTVNLS
FDPSEDVAAPYILKGAAPKMAILREQGVNSHVEMAAAFDRAGFESRDVHMSDILSGRISL
EEFQGLVACGGFSYGDVLGAGEGWAKSILFNARARDEFSRFFERDSSFALGVCNGCQMLS
NLKEIIPGSEHWPRFVRNRSERFEARFSLVEVQQSPSLFFQGMAGSRMPIAVSHGEGHAE
FASAQALALAEASGTIALRFVNGKGEIATQYPQNPNGSPNGLTGICTTDGRVTLMMPHPE
RVFRTVANSWHPDNWGEDSPWMRMFRNARVNLG