Protein Info for Shewana3_1139 in Shewanella sp. ANA-3

Name: thrA
Annotation: bifunctional aspartokinase I/homeserine dehydrogenase I (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 822 PF00696: AA_kinase" amino acids 2 to 285 (284 residues), 160.2 bits, see alignment E=1.9e-50 TIGR00657: aspartate kinase" amino acids 3 to 461 (459 residues), 418.2 bits, see alignment E=2.3e-129 PF13840: ACT_7" amino acids 394 to 457 (64 residues), 34.5 bits, see alignment 3.8e-12 PF03447: NAD_binding_3" amino acids 473 to 607 (135 residues), 88.3 bits, see alignment E=1.6e-28 PF00742: Homoserine_dh" amino acids 615 to 811 (197 residues), 160.4 bits, see alignment E=1e-50

Best Hits

Swiss-Prot: 60% identical to AK1H_SERMA: Bifunctional aspartokinase/homoserine dehydrogenase 1 (thrA) from Serratia marcescens

KEGG orthology group: K12524, bifunctional aspartokinase / homoserine dehydrogenase 1 [EC: 1.1.1.3 2.7.2.4] (inferred from 100% identity to shn:Shewana3_1139)

MetaCyc: 60% identical to fused aspartate kinase/homoserine dehydrogenase 1 (Escherichia coli K-12 substr. MG1655)
Aspartate kinase. [EC: 2.7.2.4]; Homoserine dehydrogenase. [EC: 2.7.2.4, 1.1.1.3]

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis or Methionine Biosynthesis (EC 1.1.1.3, EC 2.7.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.3, 2.7.2.4

Use Curated BLAST to search for 1.1.1.3 or 2.7.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KUA5 at UniProt or InterPro

Protein Sequence (822 amino acids)

>Shewana3_1139 bifunctional aspartokinase I/homeserine dehydrogenase I (RefSeq) (Shewanella sp. ANA-3)
MKVMKFGGTSLANWQRFSMAADIVAKAAKAEPVATVLSAPATVTNALLEMVDVAVKGEDY
SPVIQHVERVFTSLYQDAVSSGLSSSQSEVLFAGLSVQLARWQDRLRGITLLQECPDGVR
AEIVVAGERLSAALMEQVMLAKGITSAQLDPRELFLGRGRPLESVVDIAVSKPRFKNLAL
DEKRVWVMPGFTAADEDGKVVTLGRNGSDYSAAVLAACLDASSCEIWTDVDGVYNTDPRV
VTDAKLLSQLSYQEAMELSYFGAKVLHPKTIAPIAQFHIPCYIKNSFNPDAPGTLVSNQA
DESGLQVKAISNLDNQTMFDVSGPGMKGMVGMASRTLAAISRSGVSVSLITQSSCEYSIS
FCVATSDAAKVKSALEQEFELEIKSDLLEPIEMRHDLAIVSLIGDGMRTHKGVAARFFQA
LAQASVNIIAIAQGSSERSISTVIEQRKTKHAVAACHQGFFDVQQYLDVFLVGCGNVGAG
LLEQIKHQASVLKEQHISIRVCGIVNSSKMLLDSAGIDLNNWQNLLADSQQPSDLSALLA
WVKEQQLLNPVLVDCTSSDQVSNQYLEVMNAGMHVVTPNKKANTRDYAYYQALRQTALKQ
RRQFLYETNVGAGLPVIDNLKKLLFAGDKLHKFNGILSGSLSFIFGKLDEGMTLSEATKL
AREKCFTEPDPRDDLSGMDVARKVLILAREVGLKLELSDIVVDSVLPDDFDDSGDVESFM
ARLTEADAAIAARVAEAKAQGKVLRYVGQIEEGACYVRITEVDATDPLYSVKGGENALAF
YSRYYQPIPFVLRGYGAGTEVTAAGAFADVLRTLNWTREVSV