Protein Info for Shewana3_1111 in Shewanella sp. ANA-3

Annotation: (p)ppGpp synthetase I, SpoT/RelA (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 735 PF13328: HD_4" amino acids 45 to 190 (146 residues), 135.1 bits, see alignment E=6.4e-43 TIGR00691: RelA/SpoT family protein" amino acids 64 to 733 (670 residues), 623.4 bits, see alignment E=2.6e-191 PF04607: RelA_SpoT" amino acids 250 to 360 (111 residues), 145.5 bits, see alignment E=2.6e-46 PF02824: TGS" amino acids 404 to 463 (60 residues), 67.5 bits, see alignment 3e-22 PF19296: RelA_AH" amino acids 493 to 557 (65 residues), 64.6 bits, see alignment E=2.1e-21 PF28438: RelA_RIS" amino acids 591 to 652 (62 residues), 78.5 bits, see alignment 8.7e-26 PF13291: ACT_4" amino acids 658 to 734 (77 residues), 59.5 bits, see alignment E=1.5e-19

Best Hits

Swiss-Prot: 63% identical to RELA_SHIFL: GTP pyrophosphokinase (relA) from Shigella flexneri

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 100% identity to shn:Shewana3_1111)

MetaCyc: 63% identical to GDP/GTP pyrophosphokinase (Escherichia coli K-12 substr. MG1655)
GTP diphosphokinase. [EC: 2.7.6.5]; 2.7.6.5 [EC: 2.7.6.5]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KU77 at UniProt or InterPro

Protein Sequence (735 amino acids)

>Shewana3_1111 (p)ppGpp synthetase I, SpoT/RelA (RefSeq) (Shewanella sp. ANA-3)
MVSVREAHFNDPDFHLEDWVARYVSHVEEAQTLLTLIAQVEALPAKSPAAKRELLERARE
MIEILAPLNMDIETLQAAILFVVFDAGLLNEEAIKEKFGEPLARLVASVVTMDAIGALKI
NPNSRSSEPQIDNIRRMLLAMVEDVRAVVIKLAERVCLLRAVKNADEETRVLLAREIADI
YAPLANRLGIGQLKWELEDISFRYLHPDTYKDIAKQLDGKRLDREVYIEKFVEQLQQRLD
EDHIRAKVYGRPKHIYSIWRKMKGKHLKFDELFDVRAVRIVTERLQDCYGALGVVHTLWH
HIPREFDDYVANPKPNGYQSIHTVVVGPEGKTVEIQIRTQDMHEDAELGVAAHWKYKEGN
HSGKQSGYEEKINWLRKILQWQEDVVESGNLVEEVRSQVFEDRVYVFTPSGEVVDLPLGS
TVLDFAYYIHSQVGHKCIGAKVDGRIVPFTYQVETGERIEIITSKHPNPKRDWLNPNLGY
IRTSRARSKIQHWFKQQDRDKNIIAGKEMLEAELARVSLKIKDAAIAVERFNMASMDDLL
AAIGGGDVRLHQVVNHIQSKLRLDEASEEDAVEELVKKSQPKSGTNSRGQVEVNGVGNLL
SHIARCCQPVPGDEILGFITKGRGISVHRSDCEQVKELMRVHPERGVDVVWGENYSGGYR
MRLRVLAHDRSGLLRDLTSVLAAEKSNVLAMSSSSDIKNQTAAIELELELYNLDGLSRVL
SKLSQVDSVIEARRL