Protein Info for Shewana3_0991 in Shewanella sp. ANA-3

Annotation: penicillin-binding protein 6 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF00768: Peptidase_S11" amino acids 30 to 261 (232 residues), 323.6 bits, see alignment E=1.1e-100 PF13354: Beta-lactamase2" amino acids 43 to 192 (150 residues), 36.9 bits, see alignment E=3.5e-13 PF07943: PBP5_C" amino acids 281 to 371 (91 residues), 100.7 bits, see alignment E=6.3e-33

Best Hits

Swiss-Prot: 57% identical to DACA_ECO57: D-alanyl-D-alanine carboxypeptidase DacA (dacA) from Escherichia coli O157:H7

KEGG orthology group: K07258, D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5/6) [EC: 3.4.16.4] (inferred from 99% identity to son:SO_1164)

MetaCyc: 57% identical to D-alanyl-D-alanine carboxypeptidase DacA (Escherichia coli K-12 substr. MG1655)
Beta-lactamase. [EC: 3.5.2.6]; Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.5.2.6, 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.5.2.6, 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.5.2.6, 3.4.16.4, 3.4.17.8]; 3.4.16.4 [EC: 3.5.2.6, 3.4.16.4, 3.4.17.8]

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4, 3.5.2.6

Use Curated BLAST to search for 3.4.16.4 or 3.4.17.8 or 3.5.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KTV7 at UniProt or InterPro

Protein Sequence (391 amino acids)

>Shewana3_0991 penicillin-binding protein 6 (RefSeq) (Shewanella sp. ANA-3)
MMNFVKSPIKTLLLISSVSLPVYAAQPIVTPDAPTVAAKAYVLMDYYSGQIIAEENAYES
LNPASLTKMMTSYVIGQEIKAGNVSPDDDVTISKNAWSKNFSDSSKMFIEVGKTVKVSDL
NRGIIIQSGNDACVAMAEHIAGTEGAFVDMMNSWAKQLGMRDSYFENSHGLDSENHKSTA
YDMALLGAALIRDVPEEYRVYSEKYYTFNGIKQYNRNGLLWDNSMNVDGIKTGHTSGAGY
NLVASATKDGMRLISVVMGTQSEAARKAESKKLLTYGFRFFETVTPYKAGDSFVTQQIWY
GDKSTVDLGVATDTPITISRGRAKDLKANFELTKPLDAPLKKGETVGRLYFQLDGKDIAQ
FPLVTLQEVNEGSWFSKLVDYFKQLFSGWFS