Protein Info for Shewana3_0955 in Shewanella sp. ANA-3

Name: proA
Annotation: gamma-glutamyl phosphate reductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 11 to 290 (280 residues), 61.1 bits, E=3.9e-21
• TIGR00407: glutamate-5-semialdehyde dehydrogenase
  • amino acids 17 to 414 (398 residues), 547.7 bits, E=7.1e-169

Best Hits

Swiss-Prot: 94% identical to PROA_SHEON: Gamma-glutamyl phosphate reductase (proA) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00147, glutamate-5-semialdehyde dehydrogenase [EC: 1.2.1.41] (inferred from 100% identity to shn:Shewana3_0955)

Predicted SEED Role

"Gamma-glutamyl phosphate reductase (EC 1.2.1.41)" in subsystem Proline Synthesis (EC 1.2.1.41)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-ornithine biosynthesis II (3/3 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-N^δ-acetylornithine biosynthesis (5/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.41

Use Curated BLAST to search for 1.2.1.41

Sequence Analysis Tools

See A0KTS1 at UniProt or InterPro

Protein Sequence (425 amino acids)

>Shewana3_0955 gamma-glutamyl phosphate reductase (RefSeq) (Shewanella sp. ANA-3)
MSQTNQAQYLQQLGSQAKQASYALANLSAVQKADLLEAIAEALTQNTQAILAANAKDVAA
AKAEGLNDAMIDRLLLNESRLAGIIGDIGDVVRLADPVGEEFGSRVLDNGLRLTRRRVPL
GVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLESNKLISEVIRGAIASQGLPVDA
VQLIDSPDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKH
ADLERALEVIANAKVQRPTVCNALDTLLVDTAVAERFVPQIAEYLHRLGVRFSVCEQSYA
LLDGLGFDISPATEQSFATEWLSLTLGIKVVSDIDTAIAHIRTYSSGHSEAILTDDIHVA
THFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWLAWGD
YTSRA