Protein Info for Shewana3_0868 in Shewanella sp. ANA-3

Annotation: diguanylate cyclase/phosphodiesterase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 743 PF11849: DUF3369" amino acids 148 to 318 (171 residues), 130.1 bits, see alignment E=1.5e-41 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 318 to 416 (99 residues), 44.7 bits, see alignment E=5.8e-16 PF00990: GGDEF" amino acids 323 to 465 (143 residues), 55.6 bits, see alignment E=8.4e-19 PF00563: EAL" amino acids 490 to 725 (236 residues), 236.4 bits, see alignment E=4.6e-74

Best Hits

KEGG orthology group: None (inferred from 100% identity to shn:Shewana3_0868)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KTI7 at UniProt or InterPro

Protein Sequence (743 amino acids)

>Shewana3_0868 diguanylate cyclase/phosphodiesterase (RefSeq) (Shewanella sp. ANA-3)
MVSQSQEIVFLDEPIASTAVKAAKAVKILTVDDDINFQRSTAFALSTLTILGSKIELTQA
FSYAEACQIVANEDDFAIALVDVVMETEDAGLRLVRGIREVLGNEKIRIILLTGQPGMAP
VFNVMRDYDINDYWTKSELSADRLQTILTTNLRSYQQISNIANAKRGLQLIAESSGALYT
SRNIKELSSKMLQQLAILLNLPSGGIVCVKAINDLSEFGHIHQIIGASGEFESYYGKALQ
ELEQQHIRIQLELSLSSKQSRIEPQYTCLFFPGELAGNDYAVYLATGRLLDATETELIRV
FSMNICGGLHSVSLMSQLDKIAFEDPLLKIPNRNVLIRTLDGILKEDVRDKFNLLLIDID
KFSDFNHVFGSEHGNDMLKHVANSLREQFDASVLVSRLKDDKFAILGPKHLVTAALIEQL
FVAPYQQGTSCITVNISTVTLPLELAEGDAVAAITQLNMALKKAKSLGIRQHVTYQPNND
DVTSHFELLQALQLGITQGDIHIALQPQVSLQTGEVVGVEALARWTLPNGDKVPPLRFIP
IAEATGLITQLGEQLFVQSCQAMHALAEAGYQNLRIGVNLSAMQFEQEKIVNQLHSHIEA
AQIASKHIEIEVTESIAMQNFEVINKQLKALRNMNIKVAIDDFGTGFSSLAYLSKLTFDR
IKIDKSFIDNITTDDGAAAIVDSIILLGERFKMNVIAEGVETREQARWLKDRGCHDAQGY
FYAKPMPLQELLVWLANYQKSIL