Protein Info for Shewana3_0832 in Shewanella sp. ANA-3

Annotation: glutamate-ammonia ligase adenylyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 954 PF03710: GlnE" amino acids 43 to 278 (236 residues), 290 bits, see alignment E=1.5e-90 amino acids 560 to 815 (256 residues), 304.3 bits, see alignment E=6.4e-95 PF08335: GlnD_UR_UTase" amino acids 309 to 446 (138 residues), 88.5 bits, see alignment E=4.2e-29 amino acids 831 to 929 (99 residues), 38.7 bits, see alignment E=9.9e-14

Best Hits

Swiss-Prot: 95% identical to GLNE_SHEON: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 100% identity to shn:Shewana3_0832)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KTF1 at UniProt or InterPro

Protein Sequence (954 amino acids)

>Shewana3_0832 glutamate-ammonia ligase adenylyltransferase (RefSeq) (Shewanella sp. ANA-3)
MAVQKDSNKSLSPLVTQAAERHWARLAEVWSEGLANLTPAQQQELKNVLGLSDYIAAQLT
RSPEWINTLFAGDLQQVERQTFDAQLHDQLSSSTTEDLAKRQLRRFRNYQMVRLAWRDFL
DYASLEESLLDLSALAEALVIGARDWLYKEMCTQYGTPMDKAGNPQPLLILGMGKLGGRE
LNFSSDIDLIFTFPEHGETVGGRRSLDNQQFFIRMGQRLVNLLDQITVDGFVFRVDMRLR
PYGESGPLVVSFSGLEDYYQEQGRDWERYAMVKARSLGPWNHFSDELHSLLRPFVYRRYI
DFSAIESLRKMKQLIAQEVRRRQLTDNIKLGAGGIREVEFVVQSFQLIRGGREPALRQQS
LFGAMDTLYSLGQFEYLAVDELKHSYLLLRRVENLLQAIDDKQTQTLPNNPLDWARLCAV
LNMTNETDLRTHIEAAMAKIHRHFKATVGGEEGEEKAEHWTAQLWNVQQDDHAVNLLAEQ
QIDDDKLWPLLSSWRDTVTKRSIGPRGRETLDKLMPRLLDELLNQPSPSAAFEPVSKVLE
QILTRTTYLELLCENPGARQQLVSLCCASPWIAAQLAKFPMLLDELIDPAHLYDTTSLDD
YPSELRQYLLRVPEEDMEQQMEALRQFKLSQQLKIAAADVTGVLPVMQVSDHLTFLAEAI
IEQVVIQAWQQVAVRHGVPSYLEEGNDTGFAVIGYGKLGGIELGYGSDLDLVFLYEAPEN
MANSLTNGDRPIEVGHFYLKLAQRILHLFSTRTTSGELYEVDMRLRPSGSSGLMVSEIAR
FGEYQTQEAWTWEHQALVRSRFVFGDNSLAAKFSQIRASVLEQARDKDELKKAVREMRQK
MRDHLLKVSEGEFDLKQSPGGITDIEFIAQYLVLAHAHEYPELSIWSDNVRIFGVLAELE
LLPLMSAQHLTQSYCWLRDENHRLTLQQKAGKLAYADVEEHAERVMNIYRNILE