Protein Info for Shewana3_0760 in Shewanella sp. ANA-3

Annotation: ribosomal small subunit pseudouridine synthase A (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 235 PF01479: S4" amino acids 7 to 53 (47 residues), 27.8 bits, see alignment 1.7e-10 PF00849: PseudoU_synth_2" amino acids 67 to 197 (131 residues), 65.8 bits, see alignment E=5.3e-22 TIGR00093: pseudouridine synthase" amino acids 71 to 228 (158 residues), 152.1 bits, see alignment E=5.3e-49

Best Hits

Swiss-Prot: 56% identical to RSUA_ECO57: Ribosomal small subunit pseudouridine synthase A (rsuA) from Escherichia coli O157:H7

KEGG orthology group: K06183, ribosomal small subunit pseudouridine synthase A [EC: 5.4.99.12] (inferred from 100% identity to shn:Shewana3_0760)

MetaCyc: 56% identical to 16S rRNA pseudouridine516 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11833 [EC: 5.4.99.19]

Predicted SEED Role

"Ribosomal small subunit pseudouridine synthase A (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KT79 at UniProt or InterPro

Protein Sequence (235 amino acids)

>Shewana3_0760 ribosomal small subunit pseudouridine synthase A (RefSeq) (Shewanella sp. ANA-3)
MKLSRPVRLDKFICESTSLTRSLAKKALHRGDITCDGAVIKDAGFKVLEGMQVCLEGEFI
SLVGERYLMLNKPVDTICSTIDEVYPSVLSLLDIEKPETLHIAGRLDADTTGLVLITSDG
QWSHRITSPKKDCGKRYLVTLAEPVDESLIGVFAAGVELRNEDCLTKPALLEIIEPQLVR
LTITEGKYHQVKRMFAAVGNHVVGLHRESIGKIELDAELALGEWRYLTSEEIASI