Protein Info for Shewana3_0670 in Shewanella sp. ANA-3

Annotation: PTS IIA-like nitrogen-regulatory protein PtsN (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 147 TIGR01419: PTS IIA-like nitrogen-regulatory protein PtsN" amino acids 3 to 146 (144 residues), 159.7 bits, see alignment E=2.2e-51 PF00359: PTS_EIIA_2" amino acids 8 to 146 (139 residues), 121.5 bits, see alignment E=1.4e-39

Best Hits

Swiss-Prot: 56% identical to PTSN_KLEOX: Nitrogen regulatory protein (ptsN) from Klebsiella oxytoca

KEGG orthology group: K02806, PTS system, nitrogen regulatory IIA component [EC: 2.7.1.69] (inferred from 99% identity to spc:Sputcn32_0713)

Predicted SEED Role

"PTS system nitrogen-specific IIA component, PtsN"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KSY9 at UniProt or InterPro

Protein Sequence (147 amino acids)

>Shewana3_0670 PTS IIA-like nitrogen-regulatory protein PtsN (RefSeq) (Shewanella sp. ANA-3)
MELRTILRPECTTCATPGSKKKVLELISDLAAAQYPTLSSQEIFESLVAREKMGSTGIGN
GIAIPHGRLTDITQPIAILVKCEEPIAFDAIDKQPVDILFALLVPADQCQQHLSTLSCMA
EKLSDKQILKQLRKTHDETELYQVITG