Protein Info for Shewana3_0554 in Shewanella sp. ANA-3

Annotation: diguanylate cyclase/phosphodiesterase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 584 PF00563: EAL" amino acids 9 to 239 (231 residues), 177.1 bits, see alignment E=5.9e-56 PF00990: GGDEF" amino acids 404 to 500 (97 residues), 47.6 bits, see alignment E=2.4e-16 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 413 to 492 (80 residues), 43.5 bits, see alignment E=1.3e-15

Best Hits

KEGG orthology group: None (inferred from 100% identity to shn:Shewana3_0554)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KSM4 at UniProt or InterPro

Protein Sequence (584 amino acids)

>Shewana3_0554 diguanylate cyclase/phosphodiesterase (RefSeq) (Shewanella sp. ANA-3)
MTSLDLAKELDKIIAKGAISPLFQPIFNILEHKIHGFEALSRGPEHSPLYSPIPLFKTAE
HQGKLSELETLCRRISLEQFKLRQFQGRLFINISPKALLGPTHPKGMTLQLLQQLGISPS
QVVIELSEQYPADDIDLLKSCLNHYRSQGFMTAIDDLGAGYSGLRLWSELSPDYVKIDRH
FIHQIDSTPVKQEFVRSIVELCQSLTCKVIAEGIETQEELAVLKQLGIVYCQGYLLGRPE
AQPSRAMKATLVPNNTQVQPRYSESAESLCTSAITVVPTLKLKHLSDTFSSQPALQAVVV
VQDQLPLGIISRATLLELFSTPYGRALHENHAVSEVMDPQVLQIEANEPLSIVSQLLTSE
SANTVAQQFIILRRGKLLGIGHTKDLLQRITEHRIKMARHANPLTDLPGNVPIQEELKRL
RNQQKPFYLAYFDLCQFKPYNDIYGFCRGDEVICEVANLLVKYKTDNCFIGHVGGDDFVV
ISTCEQLIERCQRILTDFEANKSLFYSAEHWQAQEMLADDRQGQACYHKLISLCVGVLTP
AHTYNCNEHSLSTLSAKAKKQAKSASNGFCLLEERDISPQALSA