Protein Info for Shewana3_0523 in Shewanella sp. ANA-3

Annotation: EmrB/QacA family drug resistance transporter (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 transmembrane" amino acids 28 to 47 (20 residues), see Phobius details amino acids 67 to 87 (21 residues), see Phobius details amino acids 96 to 115 (20 residues), see Phobius details amino acids 121 to 143 (23 residues), see Phobius details amino acids 154 to 175 (22 residues), see Phobius details amino acids 183 to 204 (22 residues), see Phobius details amino acids 216 to 236 (21 residues), see Phobius details amino acids 249 to 267 (19 residues), see Phobius details amino acids 287 to 310 (24 residues), see Phobius details amino acids 322 to 341 (20 residues), see Phobius details amino acids 351 to 367 (17 residues), see Phobius details amino acids 387 to 404 (18 residues), see Phobius details amino acids 417 to 436 (20 residues), see Phobius details amino acids 500 to 521 (22 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 32 to 517 (486 residues), 415.3 bits, see alignment E=1.8e-128 PF00083: Sugar_tr" amino acids 34 to 199 (166 residues), 26.5 bits, see alignment E=3.2e-10 PF07690: MFS_1" amino acids 35 to 428 (394 residues), 163.1 bits, see alignment E=8.6e-52

Best Hits

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 100% identity to shn:Shewana3_0523)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KSJ4 at UniProt or InterPro

Protein Sequence (535 amino acids)

>Shewana3_0523 EmrB/QacA family drug resistance transporter (RefSeq) (Shewanella sp. ANA-3)
MSTIAAAPEAIVAGAIPSEYERGSRRSWIAVFGGLIGAFMAILDIQITNASMKEIQGSLG
ATLEEGSWISTAYLVAEMIAIPLSGWLSTGLSVRRYLLWTTAAFIFASILCSISWNLEAM
IAFRALQGFFGGALIPLAFRLILEFLPENKRAVGMALFGVTATFAPSIGPTLGGWLTEHF
SWHYLFYINVPPGLLVMAMLAYGLEKRPVVWDKLKNADLAGIVTMALGMGCLEVVLEEGN
RKDWFGSDLIRNLAIIAAVNLVLFVWIQLKRKDPLVNLRLLGKRDFVLSTIAYFLLGMAL
FGAIYLIPLYLSQVHDYTPLEIGEVIMWMGFPQLLVLPLVPRLMQRFDGRYLAAFGFFMF
ALSYYMNSQMTADYAGPQMIASQVVRALGQPFILVPIGMLATAHLKPHENPSASTVLNVM
RNLGGAFGIALVATLTDNLARSHLAHVKESLPAVSTQAQDYLNQSASMLQQGGSDPYTAS
MQAKVLLGQTMSQQAYIQAYNDVFLIMGAMLLIAVVAVLLIRKPQGPVAHDAVIE