Protein Info for Shewana3_0454 in Shewanella sp. ANA-3

Annotation: diguanylate cyclase with PAS/PAC sensor (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 TIGR00229: PAS domain S-box protein" amino acids 55 to 159 (105 residues), 46.2 bits, see alignment E=4.8e-16 PF08447: PAS_3" amino acids 62 to 147 (86 residues), 73.6 bits, see alignment E=1.3e-24 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 161 to 318 (158 residues), 102.1 bits, see alignment E=2.6e-33 PF00990: GGDEF" amino acids 165 to 315 (151 residues), 116.5 bits, see alignment E=1.1e-37

Best Hits

KEGG orthology group: None (inferred from 100% identity to shn:Shewana3_0454)

Predicted SEED Role

"GGDEF domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KSC7 at UniProt or InterPro

Protein Sequence (328 amino acids)

>Shewana3_0454 diguanylate cyclase with PAS/PAC sensor (RefSeq) (Shewanella sp. ANA-3)
MVADSAAQIKQLQAEIAELKRENARLTALNNRAEEKLFAALDGNGLCLWEQHVPSGNLTL
FNVKWGELLGFSREELRAHVDSWKSKLHPEDKDWVIKAFEDHVNGKADYYQVVHRMLHKD
GSITWVSDRGRIVERLPDGTPLRMMGSHIDITQEKRYELDLARLAHSDPLTNLMNRQAIA
KAFDELLQPGQRGALFFIDLDGFKALNDHHGHKFGDNLLVHVAHSLVEHSGAQAKIARLG
GDEFVIVLLSSDIEALGMLAQSLLDVYRQRFLLDGCEVAISLSIGIDIFEYGDRFTQSCD
RADAAMYRIKHQGKNGYSFSRNESSINR