Protein Info for Shewana3_0348 in Shewanella sp. ANA-3

Annotation: ATP-dependent DNA helicase RecG (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 696 PF17191: RecG_wedge" amino acids 17 to 173 (157 residues), 63.1 bits, see alignment E=8.5e-21 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 31 to 667 (637 residues), 774.8 bits, see alignment E=4e-237 PF01336: tRNA_anti-codon" amino acids 66 to 139 (74 residues), 33.8 bits, see alignment E=9.2e-12 PF04851: ResIII" amino acids 272 to 433 (162 residues), 49.5 bits, see alignment E=1.7e-16 PF00270: DEAD" amino acids 276 to 438 (163 residues), 93 bits, see alignment E=6.4e-30 PF00271: Helicase_C" amino acids 506 to 590 (85 residues), 66.7 bits, see alignment E=7.3e-22 PF19833: RecG_dom3_C" amino acids 620 to 678 (59 residues), 46.4 bits, see alignment 1.1e-15

Best Hits

Swiss-Prot: 66% identical to RECG_ECO57: ATP-dependent DNA helicase RecG (recG) from Escherichia coli O157:H7

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 100% identity to shn:Shewana3_0348)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KS22 at UniProt or InterPro

Protein Sequence (696 amino acids)

>Shewana3_0348 ATP-dependent DNA helicase RecG (RefSeq) (Shewanella sp. ANA-3)
MSANDLQQLDLVPITDLKGVAKRVAEKLAKLGITTVQDLLFHLPLRYEDRTQIYPIAALM
PGNYGTIEAEIQSTQIIQGRKRMLVCNVRDNTGSMSLRFFNFSMAQRNAMQNGLMIRAYG
EIRRGNHQAEIVHPEYKIVYPGEDIHLSDTLTPIYPTTEGLKQASWIKLTEQALELLEDG
GLTELLPANLQPNSMSLKQALQTLHRPHAGISQFDLELGQHPAQQRLVQEELLAHNLSML
RLRQRSNLDAAVTMHASGQLLNPFLASLPFKPTGAQQRVVAEIGKDLAQPHPMMRLVQGD
VGSGKTLVAALAALQAIENGYQVAMMAPTELLAEQHATNFAAWFEPLGLKVGWLAGKLKG
KARAQSLEDIESGAAQIVIGTHAIFQQQVTFNKLALIIIDEQHRFGVHQRMGLREKGINQ
GFHPHQLIMTATPIPRTLAMTAYADLDTSIIDELPPGRTPVTTVAIPDSRRNEVLERVRN
SVVTDKRQAYWVCTLIEESEVLECQAAEDTAEELRQALPELSIGLVHGRMKSAEKQKIMA
DFKAGSIHLLVATTVIEVGVDVPNSSLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLL
YKAPLSQTASQRLNVLRQSNDGFVIAQKDLEIRGPGEVLGTKQTGLAELKIADLVRDQAL
IPHIQKLASHVMSQAPESVDAIIHRWLGHRQQYVQA