Protein Info for Shewana3_0175 in Shewanella sp. ANA-3

Annotation: tRNA 2-selenouridine synthase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 365 PF00581: Rhodanese" amino acids 11 to 128 (118 residues), 22.2 bits, see alignment E=7.8e-09 TIGR03167: tRNA 2-selenouridine synthase" amino acids 19 to 347 (329 residues), 357 bits, see alignment E=5.2e-111

Best Hits

Swiss-Prot: 100% identical to SELU_SHESA: tRNA 2-selenouridine/geranyl-2-thiouridine synthase (selU) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K06917, tRNA 2-selenouridine synthase [EC: 2.9.1.-] (inferred from 100% identity to shn:Shewana3_0175)

MetaCyc: 47% identical to tRNA 2-selenouridine synthase (Escherichia coli K-12 substr. MG1655)
RXN0-2281 [EC: 2.9.1.3]

Predicted SEED Role

"Selenophosphate-dependent tRNA 2-selenouridine synthase" in subsystem Selenocysteine metabolism

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.9.1.- or 2.9.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KRK0 at UniProt or InterPro

Protein Sequence (365 amino acids)

>Shewana3_0175 tRNA 2-selenouridine synthase (RefSeq) (Shewanella sp. ANA-3)
MTTKLIPAQQYHDIFIAGQPLIDLRAPIEFDRGAFPSSVNLPLMVDKEREKVGTCYKAQG
QQAAIALGHSLVHGVVKQQRIDAWLNFLSANPEAYLYCFRGGLRSQLTQQWLQEAGVTAP
YVQGGYKGMRQYLIGVIETAPSQQPLLSLSGMTGSGKTDFLRLRKEAIDLEGIANHRGSS
FGKNIDPQPTQINFENQLAIALLRHQLGNHACLLLEDESFLIGRSALPQSFYSAMQTADI
VVLEEDDDIRLTRLLDEYVHKMHLGFSERLGLEAGFEAFSHYLLQSLGSIRKRLGGKQYQ
ELQDMMQQALSQQLNQNQTSQHMAWISLLLHKYYDPMYEYQLQKKAGNILFRGSHQAMHE
WLDNY