Protein Info for Shewana3_0127 in Shewanella sp. ANA-3

Annotation: putative SAM-dependent methyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 364 PF05971: Methyltransf_10" amino acids 50 to 348 (299 residues), 385.7 bits, see alignment E=8e-120

Best Hits

Swiss-Prot: 100% identical to RLMF_SHESA: Ribosomal RNA large subunit methyltransferase F (rlmF) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K06970, ribosomal RNA large subunit methyltransferase F [EC: 2.1.1.181] (inferred from 100% identity to shn:Shewana3_0127)

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51)" (EC 2.1.1.51)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.51

Use Curated BLAST to search for 2.1.1.181 or 2.1.1.51

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KRF2 at UniProt or InterPro

Protein Sequence (364 amino acids)

>Shewana3_0127 putative SAM-dependent methyltransferase (RefSeq) (Shewanella sp. ANA-3)
MPKPAIKTAAKLAMSSAGKRGKPSTPKSLAKPQTTKPKTASKLKAKHGEQKRLHPRNLHI
NGYDFPALMASFPKLKAFVRPTPYGALSIDFADPFAVKNLNAALLKHHYGLAFWDIPKGA
LCPPIPGRVDYLHYLADLLFEGAKVKRAAAIHALDIGTGANGVYAILGHQVYDWQFVASD
INPQSLINVQRIIDNNPSLQGHLSLRRQQDDKAVFKGIIQASDRFELTLCNPPFHGSLKE
ASEGSLRKVRNLQLNRGEQPKATSATLNFGGQAAELWCQGGEKQFLATMIRESQAFAEQC
LWFTSLVSKQENLKPCYQALEKLGVDTVKTIEMQQGNKVTRVLAWSFHSQAKRLQWRNQV
ISGA