Protein Info for Shewana3_0101 in Shewanella sp. ANA-3

Annotation: histidine ammonia-lyase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 513 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR01225: histidine ammonia-lyase" amino acids 8 to 511 (504 residues), 714.3 bits, see alignment E=3.5e-219 PF00221: Lyase_aromatic" amino acids 14 to 474 (461 residues), 634.2 bits, see alignment E=6.2e-195

Best Hits

Swiss-Prot: 98% identical to HUTH_SHEON: Histidine ammonia-lyase (hutH) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 100% identity to shn:Shewana3_0101)

MetaCyc: 68% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]

Predicted SEED Role

"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.3

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KRC7 at UniProt or InterPro

Protein Sequence (513 amino acids)

>Shewana3_0101 histidine ammonia-lyase (RefSeq) (Shewanella sp. ANA-3)
MKSVNHLVLTPGSLSLAQLREISRHKLTLELAPEAINDINTSAQIVQKVLDEGRTVYGIN
TGFGLLANTKIAPEDLQLLQRSIVLSHAAGTGQYMQDATVRLMMVLKINSLSRGFSGIRL
EVINFLISLVNAEVYPCVPEKGSVGASGDLAPLAHMCLPLLGEGEMSYQGQIISAAEGLE
IAGLKPIDLAAKEGLALLNGTQASTALALEGLFHAEDLFAASSVIGAMSVEAAMGSRSPF
DPRIHAARGQKGQIDAAMVFRHLLGEESEISLSHINCEKVQDPYSLRCQPQVLGACLTQI
RQAAEVLGTEANGVTDNPLVFQDTGDIISGGNFHAEPVAMAADNLAIAIAELGAIAERRI
ALLIDSSLSKLPPFLVKNGGVNSGFMIAQVTAAALASENKTYAHPASVDSLPTSANQEDH
VSMATFAARRLRDMSENTRGVLAVELLAAAQGLDFRAPLMPSKAVAQAKAELREVVAYYD
KDRYFAPDIDAATDLLYTASFNAYLPQGVLPSL