Protein Info for Shewana3_0089 in Shewanella sp. ANA-3

Annotation: 2,3-diketo-5-methylthio-1-phosphopentane phosphatase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00702: Hydrolase
  • amino acids 3 to 197 (195 residues), 41.4 bits, E=1.1e-14
• TIGR01691: 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
  • amino acids 3 to 214 (212 residues), 237.7 bits, E=1.1e-74
• TIGR01549: HAD hydrolase, family IA, variant 1
  • amino acids 116 to 197 (82 residues), 28.9 bits, E=1.3e-10

Best Hits

Swiss-Prot: 100% identical to MTNC_SHESA: Enolase-phosphatase E1 (mtnC) from Shewanella sp. (strain ANA-3)

KEGG orthology group: K09880, enolase-phosphatase E1 [EC: 3.1.3.77] (inferred from 99% identity to she:Shewmr4_0090)

MetaCyc: 42% identical to acireductone synthase [multifunctional] (Klebsiella oxytoca)
Acireductone synthase. [EC: 3.1.3.77, 5.3.2.5]; R83-RXN [EC: 3.1.3.77, 5.3.2.5, 3.1.3.87]; 3.1.3.77 [EC: 3.1.3.77, 5.3.2.5, 3.1.3.87]

Predicted SEED Role

"2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatase related protein"

MetaCyc Pathways

• L-methionine salvage cycle III (6/11 steps found)
• L-methionine salvage cycle I (bacteria and plants) (6/12 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation I (2/7 steps found)
• L-methionine salvage cycle II (plants) (4/11 steps found)

KEGG Metabolic Maps

• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.77 or 3.1.3.87 or 5.3.2.5

Sequence Analysis Tools

See A0KRB5 at UniProt or InterPro

Protein Sequence (226 amino acids)

>Shewana3_0089 2,3-diketo-5-methylthio-1-phosphopentane phosphatase (RefSeq) (Shewanella sp. ANA-3)
MGIRAIVVDTAGTTTDLTFIQDVLFPYSVKALPDFLAQNQHNVLVENCICDTRDIALEPD
ANLARVTEILQQWVHEDRKATPLKTLQGLIWKQGYAHGEFTGHIFPDFIEAVNRFSAQKL
RIYSFSSGSVEAQKLLFSHSDGGDLTEMFSGHFDTRTGNKLDKQAYANILNTISLSPKQV
LFVSDVVEELKAAEAAGMMTCQMVRDSKQRTGDYRTINSFDELVID