Protein Info for Shewana3_0080 in Shewanella sp. ANA-3

Annotation: AMP-dependent synthetase and ligase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 13 to 425 (413 residues), 255.1 bits, E=1e-79
• PF23562: AMP-binding_C_3
  • amino acids 466 to 596 (131 residues), 34 bits, E=3.5e-12

Best Hits

KEGG orthology group: K01897, long-chain acyl-CoA synthetase [EC: 6.2.1.3] (inferred from 100% identity to she:Shewmr4_0078)

Predicted SEED Role

"Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)" in subsystem Biotin biosynthesis or n-Phenylalkanoic acid degradation (EC 6.2.1.3)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (50/53 steps found)
• oleate β-oxidation (32/35 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• superpathway of fatty acid biosynthesis II (plant) (37/43 steps found)
• fatty acid salvage (6/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• long-chain fatty acid activation (1/1 steps found)
• palmitate biosynthesis III (21/29 steps found)
• phytol degradation (3/4 steps found)
• stearate biosynthesis IV (4/6 steps found)
• γ-linolenate biosynthesis II (animals) (1/2 steps found)
• linoleate biosynthesis II (animals) (1/2 steps found)
• phosphatidylcholine acyl editing (2/4 steps found)
• alkane biosynthesis II (1/3 steps found)
• oleate biosynthesis I (plants) (1/3 steps found)
• 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
• octane oxidation (2/5 steps found)
• long chain fatty acid ester synthesis (engineered) (1/4 steps found)
• wax esters biosynthesis II (1/4 steps found)
• 6-gingerol analog biosynthesis (engineered) (2/6 steps found)
• 2-deoxy-D-ribose degradation II (3/8 steps found)
• ceramide degradation by α-oxidation (2/7 steps found)
• sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
• stearate biosynthesis I (animals) (1/6 steps found)
• arachidonate biosynthesis III (6-desaturase, mammals) (1/7 steps found)
• capsaicin biosynthesis (1/7 steps found)
• icosapentaenoate biosynthesis II (6-desaturase, mammals) (1/7 steps found)
• icosapentaenoate biosynthesis III (8-desaturase, mammals) (1/7 steps found)
• ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
• sporopollenin precursors biosynthesis (5/18 steps found)
• cutin biosynthesis (2/16 steps found)
• suberin monomers biosynthesis (3/20 steps found)

KEGG Metabolic Maps

• Fatty acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.3

Use Curated BLAST to search for 6.2.1.3

Sequence Analysis Tools

See A0KRA6 at UniProt or InterPro

Protein Sequence (597 amino acids)

>Shewana3_0080 AMP-dependent synthetase and ligase (RefSeq) (Shewanella sp. ANA-3)
MSLEQYHVIRLLQQQSQSLKEAIALEGFEMAAPWHQVSWQAFDQISHKIAQVLIELGVQV
QDRCVILSQNCPQWTCADIGTLKSRAVVVPIYPTSTLEQASFIINDAAAKVIFVDDAKQY
ALACELQKQCPTLEHVIVFDASVELAQDKAQHQHLDSLLAKEYGQSAELEQRLKDANLDD
LLTLIYTSGTTGDPKGVMLDYRNMASTVRQHDQILPFTTGDVSLAFLPLSHVFERGWSFY
VLCRGGHNVYLQNTQRVKEAISAVRPHTLCVVPRFLEKVYSAVQDKVAKSAEGRKKLFAW
AMGVGERQFEVSQGRAKGGLWLSLQWRLAHKLVYSKLQAVLGGRLKFMPCGGAALDLNVA
SFFHAIGIPVLCGYGMTETNATVTCNTLDNRVAGSNGKVLPEIEVKLGKDDEILVRGDTV
MRGYYNRPEDTAAAFEDGWLKTGDAGRLDANGNLFITDRIKELMKTSNGKYIAPQRVEGT
VGRCPFIEQVAVIADARNYVTALIVPAFESLEAWAKDKGLKYESPIELLRHSHVVEHFEQ
RLKHLQQELAGFEQIKKFTLLPEAFSMEAGLITPTLKLRRKMIYHKYAHEINAMYNN