Protein Info for Shewana3_0069 in Shewanella sp. ANA-3

Updated annotation (from data): Fructokinase (EC 2.7.1.4)
Rationale: Specifically important for utilizing Sucrose. Automated validation from mutant phenotype: the predicted function (FRUCTOKINASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: ribokinase-like domain-containing protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00294: PfkB
  • amino acids 2 to 314 (313 residues), 168.8 bits, E=9.1e-54

Best Hits

KEGG orthology group: K00847, fructokinase [EC: 2.7.1.4] (inferred from 100% identity to shn:Shewana3_0069)

Predicted SEED Role

"Fructokinase (EC 2.7.1.4)" in subsystem Fructose utilization or Mannitol Utilization or Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization or Sucrose utilization or Sucrose utilization Shewanella (EC 2.7.1.4)

MetaCyc Pathways

• heterolactic fermentation (15/18 steps found)
• sucrose degradation IV (sucrose phosphorylase) (4/4 steps found)
• sucrose degradation II (sucrose synthase) (4/5 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• sucrose degradation III (sucrose invertase) (3/4 steps found)
• D-sorbitol degradation I (2/3 steps found)
• mannitol cycle (3/5 steps found)
• sucrose degradation I (sucrose phosphotransferase) (1/3 steps found)
• sucrose degradation VII (sucrose 3-dehydrogenase) (1/4 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism
• Starch and sucrose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.4

Sequence Analysis Tools

See A0KR95 at UniProt or InterPro

Protein Sequence (319 amino acids)

>Shewana3_0069 Fructokinase (EC 2.7.1.4) (Shewanella sp. ANA-3)
MTVLLSFGEVLVDLLPTDISGKSHQAIAGGAPANVAVGYAKLGGKSYFAGGISTDDYGAM
LKQALANEGVVTDYLTQVPEAPTATVVVNLDEHGERTFEFNRQGTADLCYSPRHFDAIPW
QQMDIFHLCSNTFTEASIFNASLYGACCANSFNKIVSFDVNLRLSLWPDTALLAERVEQC
FRYTQVLKMSREEAEYLALTRNKSFEDYLQFCLAHGVEVILITDGANPVQCITVNERFSV
PVPQIKAVDTTAAGDSFMAGFLFALGFELAFDLEHLTLKHRLACQAQLRKAIEFAVRCGA
VTCGQKGAFPALPTLSQVS