Protein Info for Shewana3_0066 in Shewanella sp. ANA-3

Annotation: sucrose phosphorylase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 TIGR03852: sucrose phosphorylase" amino acids 3 to 468 (466 residues), 438.7 bits, see alignment E=1.2e-135 PF00128: Alpha-amylase" amino acids 36 to 226 (191 residues), 39.7 bits, see alignment E=1.8e-14

Best Hits

Swiss-Prot: 59% identical to SUCP_AGRVI: Sucrose phosphorylase from Agrobacterium vitis

KEGG orthology group: K00690, sucrose phosphorylase [EC: 2.4.1.7] (inferred from 100% identity to shn:Shewana3_0066)

MetaCyc: 51% identical to sucrose phosphorylase (Bifidobacterium longum)
Sucrose phosphorylase. [EC: 2.4.1.7]

Predicted SEED Role

"Sucrose phosphorylase (EC 2.4.1.7)" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Sucrose utilization or Sucrose utilization Shewanella (EC 2.4.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0KR92 at UniProt or InterPro

Protein Sequence (494 amino acids)

>Shewana3_0066 sucrose phosphorylase (RefSeq) (Shewanella sp. ANA-3)
MKNQVQLITYVDRITGAGLKQLKALLDNELDGLFGGVHLLPFYYPIDGSDAGFDPIDHVQ
VDSRLGDWDDVKSIGEAFDIMADLIVNHMSAESPEFKDVLKHGKQSVYWDLFLTKDKVFP
EGLSEQDQKAIYRPRPGSCFSTYTLADGSTEEFWTTFTRNQIDIDVNSAAGKDYLNRVLA
RFNHSKVNLIRLDAAGYAIKKPGTSCFMIEESFEFVDKLAKQANELGMTTLAEIHSHHMT
QIDIAKRVDMVYDFALPPLILHTLFSQDCSALSHWLDMAPRNCITVLDTHDGIGIIDVGP
MDGLRGLLNEKQIDNLVETIHGNSQGESLQATGAAASNVDLYQINCTYYDALGQNDLDYL
MARAIQFFAPGIPQVYYTGLLAIPNDMTLLNQTHVGRDINRPYLNREKVQQALQKPVVKA
LIQLIKLRNNSAAFNGRFSMAASGKTLTLTWSAQNASASLSLDFAHKQGLITLLDEQGIE
TQIALADLLTQAQA