Protein Info for Shewana3_4297 in Shewanella sp. ANA-3

Annotation: copper/silver efflux system outer membrane protein CusC (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01845: efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family" amino acids 13 to 458 (446 residues), 352.3 bits, see alignment E=2.1e-109 PF02321: OEP" amino acids 67 to 248 (182 residues), 68.3 bits, see alignment E=3.9e-23 amino acids 272 to 456 (185 residues), 105.7 bits, see alignment E=1.4e-34

Best Hits

Swiss-Prot: 95% identical to SILC_SALTM: Probable outer membrane lipoprotein SilC (silC) from Salmonella typhimurium

KEGG orthology group: K07796, Cu(I)/Ag(I) efflux system outer membrane protein CusC (inferred from 100% identity to shn:Shewana3_4297)

MetaCyc: 70% identical to copper/silver export system outer membrane channel (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-90; TRANS-RXN0-280

Predicted SEED Role

"Cation efflux system protein CusC precursor" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L381 at UniProt or InterPro

Protein Sequence (461 amino acids)

>Shewana3_4297 copper/silver efflux system outer membrane protein CusC (RefSeq) (Shewanella sp. ANA-3)
MFKLKLLSISTIFILTGCVSLAPEYQRPAAPVPQQFSLSRNSLMPAVNGYQDTGWRNFFV
DPQVTRLIGEALANNRNLRMAALKVEEARAQFNVTDADRYPQLNASSGITYSGGLKGDKP
TTQEYDAGLELRYELDFFGKLKNLSDADRQNYFASEEARRAVHILLVSSVSQSYFSQQLA
YEQLRIARETLKNYEQSYAFVEQQLVTGSTNVLALEQARGQIESTRAEIAKREGDLAQAN
NALQLVLGTYRALPPEKGMKGGETAPVKLPSNLSSQILLQRPDIMEAEYQLKAADANIGA
ARAAFFPSITLTSGLSASSTELSSLFTPGSGMWNFIPKIDIPVFNAGRNKANLKLAEIRQ
QQSVVNYEQKIQSAFKDVSDTFALRDSLSQQLESQQRYLDSLQITLQRARGLYASGAVSY
IEVLDAERSLFATQQTILDLTYSRQVNEINLFTALGGGWVE