Protein Info for Shewana3_4295 in Shewanella sp. ANA-3

Annotation: sensor kinase CusS (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 491 transmembrane" amino acids 12 to 36 (25 residues), see Phobius details amino acids 188 to 207 (20 residues), see Phobius details PF21085: CusS" amino acids 8 to 181 (174 residues), 155.2 bits, see alignment E=3.9e-49 TIGR01386: heavy metal sensor kinase" amino acids 9 to 480 (472 residues), 628.1 bits, see alignment E=5e-193 PF00672: HAMP" amino acids 208 to 257 (50 residues), 34.9 bits, see alignment 4e-12 PF00512: HisKA" amino acids 263 to 327 (65 residues), 48.3 bits, see alignment E=2.2e-16 PF02518: HATPase_c" amino acids 372 to 481 (110 residues), 90.6 bits, see alignment E=2.3e-29 PF14501: HATPase_c_5" amino acids 380 to 469 (90 residues), 32.2 bits, see alignment E=2.2e-11

Best Hits

Swiss-Prot: 92% identical to SILS_SALTM: Probable sensor kinase SilS (silS) from Salmonella typhimurium

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 100% identity to shn:Shewana3_4295)

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L379 at UniProt or InterPro

Protein Sequence (491 amino acids)

>Shewana3_4295 sensor kinase CusS (RefSeq) (Shewanella sp. ANA-3)
MHSKPSRHPFSLSLRLTFFISLSTILAFIAFTWFMLHSVENHFAEQDVSDLQQISTTLNR
ILQSPEDPDEKKVSKIKESIASYRNVALLLLNPRGEVLFSSAQGAALRPAVNSADFSEHS
HARDVFLWTVEDPARPMDTGSEMKMETYRIIASSGQAIFQGKQQNYVMLTGLSINFHLHY
LDALKKNLIAIAVVISLLIVLIIRIAVRQGHLPLRNVSNAIKSITSENLDTRLEPTRVPI
ELEQLVISFNHMIGKIEDVFTRQANFSADIAHEIRTPITNLVTQTEIALSQDRTQKELED
VLYSSLEEYNRMTKMVSDMLFLAQADNNQLIPDRVRFDLRAEVMKVFEFFEAWAEERNIT
LKFNGMPCLVEGDPQMFRRAINNLLSNALRYTPEGQAITVSIREQESFFDLVIENPGKPI
PEEHLSRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVEAHHGRVQVESDVRSTRFILSVP
RLEQRIPEPQH