Protein Info for Shewana3_4164 in Shewanella sp. ANA-3

Annotation: HsdR family type I site-specific deoxyribonuclease (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1076 PF04313: HSDR_N" amino acids 5 to 206 (202 residues), 75.8 bits, see alignment E=1.1e-24 TIGR00348: type I site-specific deoxyribonuclease, HsdR family" amino acids 8 to 757 (750 residues), 335.9 bits, see alignment E=2.8e-104 PF04851: ResIII" amino acids 263 to 449 (187 residues), 100 bits, see alignment E=3.4e-32 PF18766: SWI2_SNF2" amino acids 267 to 484 (218 residues), 162.6 bits, see alignment E=2.9e-51 PF22679: T1R_D3-like" amino acids 581 to 740 (160 residues), 150.7 bits, see alignment E=1.1e-47 PF12008: EcoR124_C" amino acids 764 to 1056 (293 residues), 103.6 bits, see alignment E=3.9e-33

Best Hits

KEGG orthology group: K01153, type I restriction enzyme, R subunit [EC: 3.1.21.3] (inferred from 63% identity to bav:BAV0385)

Predicted SEED Role

"Type I restriction-modification system, restriction subunit R (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L2T9 at UniProt or InterPro

Protein Sequence (1076 amino acids)

>Shewana3_4164 HsdR family type I site-specific deoxyribonuclease (RefSeq) (Shewanella sp. ANA-3)
MTTFKTEAQFEQAFIEVLTHKGWEPEILKNKTEADLLQNWANILFENNRQQDRLNDVPLT
ATEMQQIIEQIKELKTPLKLNGLINGKTVAIKRDNPADALHLGKEVSLKIYDRQEIAAGQ
SRYQIVQQPKFERGSPLRNDRRGDVLLLINGMPVIHVELKRSGVPVSQAVNQIEKYSKEG
IFSGLFSLIQVFVAMEPNEAKYFANPGLDGKFNPDYQFNWADFNNEPMNHWKDIASTLLS
IPMAHQLIGFYTVADDTDGVLKVMRSYQYYAANAISDKVAKTNWQQLGSAANNPDRLGGY
VWHTTGSGKTMTSFKSAQLIAQSKDADKVIFLMDRIELGTQSLAEYRNFAGDGEDVQATE
NTHVLVTKLKSTSPADTLIVSSIQKMSNIFEEVDDEGTATNSADIEKIRAKRLVFIIDEA
HRSTFGDMLIIIKRTFPRALFFGFTGTPIQEENEKNGNTTSTVFGNELHRYSIADGIRDG
NVLGFDPYKVPTFRDGDLRQAVALEQAKSGSVAEAMDNPAKKKKFNYFMNEVPMAGYKDS
TGKYHKGIEDYVPKSQYLSETHQAKVVEDILHKWDVLSQGNKFHAILATNSIAEAIDYYR
LLKAAKPDLKVSALFDPNIDNDGSGDRGPTFKGDGLDEIMRDYNARYGHDFDFARHAAFK
KDLAARLAHKKPYERIHTDPTKQLDLLIVVDQMLTGFDSKWLNVLYLDKVIKYQNIIQAF
SRTNRLFGPDKPHGVIRYYRYPHTMEQHINDAVKLYSGDRPIGLFVDRLESNLEAMNELF
GDITELFISAGVTNFEKLPEDVEACAKFAELFKVFNQHLEAAKIQGLHWEQSVYTFTTNA
STGENGTEHEVTLAINEQTYLSLALRYKELASKGDGGGAGGGGVPFDISGYLTEIDTGKI
DADYMNSRFDKYLKELNQHNDQASIQLTLNELHKSFSSLTQAEQKYAKLFLHDLQRGDAK
LIEGHTFRDYINTYKDNAENAQINAIVNALGLDKSQLISLLNDSVNEKHLNNFGRFDALK
DTVDKAKAKVYFEKLDGVTIPPFKLNIRIDQFLKQFIFAQADDLLNEIYEDVTEMI