Protein Info for Shewana3_4145 in Shewanella sp. ANA-3

Annotation: copper-translocating P-type ATPase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 778 transmembrane" amino acids 129 to 146 (18 residues), see Phobius details amino acids 158 to 175 (18 residues), see Phobius details amino acids 195 to 214 (20 residues), see Phobius details amino acids 226 to 245 (20 residues), see Phobius details amino acids 376 to 399 (24 residues), see Phobius details amino acids 405 to 427 (23 residues), see Phobius details amino acids 721 to 740 (20 residues), see Phobius details amino acids 746 to 767 (22 residues), see Phobius details PF00403: HMA" amino acids 41 to 98 (58 residues), 58.3 bits, see alignment 1.7e-19 TIGR01511: copper-translocating P-type ATPase" amino acids 175 to 771 (597 residues), 606.1 bits, see alignment E=1.5e-185 TIGR01525: heavy metal translocating P-type ATPase" amino acids 193 to 770 (578 residues), 623.2 bits, see alignment E=1e-190 TIGR01494: HAD ATPase, P-type, family IC" amino acids 240 to 738 (499 residues), 286.7 bits, see alignment E=5.3e-89 PF00122: E1-E2_ATPase" amino acids 261 to 360 (100 residues), 106.9 bits, see alignment E=8.4e-35 PF00702: Hydrolase" amino acids 458 to 674 (217 residues), 112.4 bits, see alignment E=7.3e-36

Best Hits

Swiss-Prot: 52% identical to COPA_SALTI: Copper-exporting P-type ATPase (copA) from Salmonella typhi

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 100% identity to shn:Shewana3_4145)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0L2W8 at UniProt or InterPro

Protein Sequence (778 amino acids)

>Shewana3_4145 copper-translocating P-type ATPase (RefSeq) (Shewanella sp. ANA-3)
MSSKNSGATNVASRDGGKSTRCHEHHAHKAFHHEQTDVVELIIEGASCASCVGKIESALK
NVPGVQNAVMNFAQRTVSVTGAAQADVLVKAVDKAGYHAKLALAEREDDVLVEKERADRA
YYKRLMREMTIALSIGGPLMIYGLFVGDMTVSSAEERFVWLVVGLLTLGVMVFSGKHFYM
GAWQSFKNHSANMDTLIALGTGTAWIYSMVVVFIPDVVPEMARHVYFEATAMIIGLIDLG
LALELRARGRTSEAIKRLIGLQAKTARVIRNEKEVDIAIEQVLMDDVVRVRPGEKVPVDG
EVIEGYTAIDESMLTGEPMPVEKAVGDMVAAGTINKNGAILFRTTRIGKDTALAQIINMV
KRAQNSKPSIGRLADVIAAYFVPVVMIVAVSSALIWLNFGPNPTVAYAIVSATTVLIIAC
PCALGLATPMSVMVGVGKAAEVGVLIRNGEALQTASKISAMILDKTGTITLGTPKVTDVL
VAGKQSEDDILQLAASLESGSEHPLALAIVESAQEKGLELGKVSNFNAIAGHGVQAEVDG
NQLLFGNEKLMLECNVELGDFVERAQALAAEAKTPMYFAVNNQLAAIIAVADPIKEDSIS
AIKRLQHNGIRVVMLTGDNRATAKAVAEKAGINEFFAEVLPEDKSKKVKELQMAGEIVGM
TGDGINDAPALAIANVGFAIGTGTDVAIESADITLMRGSLHGLADAIAVSKATLRNIKQN
LLGAFIYNVAGIPFAAGALYPLFGLLLSPVIAGAAMAFSSLTVVTNANRLRLFSAREH