Protein Info for Sama_3639 in Shewanella amazonensis SB2B

Annotation: glucosamine--fructose-6-phosphate aminotransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01135: glutamine-fructose-6-phosphate transaminase (isomerizing)
  • amino acids 2 to 609 (608 residues), 872.9 bits, E=6e-267
• PF13522: GATase_6
  • amino acids 64 to 182 (119 residues), 75 bits, E=1.1e-24
• PF13537: GATase_7
  • amino acids 81 to 197 (117 residues), 60.2 bits, E=4.2e-20
• PF01380: SIS
  • amino acids 290 to 416 (127 residues), 113.3 bits, E=1.4e-36
  • amino acids 462 to 591 (130 residues), 90.6 bits, E=1.5e-29

Best Hits

Swiss-Prot: 91% identical to GLMS_SHEON: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 100% identity to saz:Sama_3639)

MetaCyc: 70% identical to L-glutamine--D-fructose-6-phosphate aminotransferase (Escherichia coli K-12 substr. MG1655)
Glutamine--fructose-6-phosphate transaminase (isomerizing). [EC: 2.6.1.16]

Predicted SEED Role

"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• CMP-legionaminate biosynthesis I (4/10 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (10/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.16

Use Curated BLAST to search for 2.6.1.16

Sequence Analysis Tools

See A1SBT5 at UniProt or InterPro

Protein Sequence (609 amino acids)

>Sama_3639 glucosamine--fructose-6-phosphate aminotransferase (RefSeq) (Shewanella amazonensis SB2B)
MCGIVGAVAQRDVAEILLEGLRRLEYRGYDSAGMAVIQNAEMNRTRRVGKVQELADALAE
APLTGGTGIAHTRWATHGEPSERNAHPHQSEGDIAVVHNGIIENHAKLRERLKGLGYQFN
SDTDTEVICHLVHHELKSADTLLAAVQATVKQLEGAYGTVVIDRRDAERMVVARSGSPLV
IGFGLGENFVASDQLALLPVTRTFAFLEEGDVAEVTRRNVRIFDVDGNEVEREAKESEVT
HDAGDKGEYRHYMLKEIYEQPRALARTLEGRIANMQVLDSAFGDKAADLLKDIKHVQIIA
CGTSYHAGMTARYWLEQWAGVSCNVEIASEFRYRKSHLFPNSLLVTISQSGETADTLAAL
RLAKEMGYQATLTICNAPGSSLVRESDMAYMMKAGAEIGVASTKAFTVQLAGLLMLTAVI
GRNNGMSAELQSSLTQSLQSLPAKVEQALGLDDAIASLAEDFADKHHALFLGRGDQYPIA
MEGALKLKEISYIHAEAYASGELKHGPLALIDADMPVIVVAPNNELLEKLKSNVEEVRAR
GGLMYVFADKDAEFASDDTMKVIPVPHCDEFMAPLIYTIPLQLLSYHVALIKGTDVDQPR
NLAKSVTVE