Protein Info for Sama_3607 in Shewanella amazonensis SB2B

Annotation: formate dehydrogenase, C subunit, putative (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 72 to 97 (26 residues), see Phobius details amino acids 118 to 143 (26 residues), see Phobius details amino acids 164 to 183 (20 residues), see Phobius details amino acids 195 to 208 (14 residues), see Phobius details amino acids 224 to 245 (22 residues), see Phobius details amino acids 257 to 281 (25 residues), see Phobius details TIGR01583: formate dehydrogenase, gamma subunit" amino acids 109 to 314 (206 residues), 166.6 bits, see alignment E=3.7e-53 PF01292: Ni_hydr_CYTB" amino acids 111 to 292 (182 residues), 76.8 bits, see alignment E=9.3e-26

Best Hits

KEGG orthology group: K00127, formate dehydrogenase, gamma subunit [EC: 1.2.1.2] (inferred from 100% identity to saz:Sama_3607)

Predicted SEED Role

"Formate dehydrogenase -O, gamma subunit (EC 1.2.1.2)" in subsystem Anaerobic respiratory reductases or Formate hydrogenase (EC 1.2.1.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1SBQ3 at UniProt or InterPro

Protein Sequence (323 amino acids)

>Sama_3607 formate dehydrogenase, C subunit, putative (RefSeq) (Shewanella amazonensis SB2B)
MNNWFKILAVAVLLMLGMGLGHAADANAQLGDAQMWAELKDGAVGYTTSTSEFHGQPINT
YDPRLLEARGTWLAPGLMAALFGMIVVFALFIMVNGISRLHHGFSGKMVYRWSKLDVSIH
WLGAIPCLLLILTGLVLLAGRFFFQPWLGEGVWAGLVYAAKQVHDVMAIPFMIGWALMTL
MWAKNQLPKMYDIKWFMVVGGYINFGPFKNQHPDAGFANAGEKMWFWTFALFGLVISASG
MLLLFPNLFEPSRTLSLTALILHGISAIIICAFSIVHIFMATVMSEGGMECMVSGYCDEN
WATQHHNLWFDEIKANGTLKYKD