Protein Info for Sama_3554 in Shewanella amazonensis SB2B
Annotation: AcrB/AcrD/AcrF family protein (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 66% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)
KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 68% identity to abo:ABO_0964)MetaCyc: 61% identical to multidrug efflux pump RND permease MdtF (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-352; TRANS-RXN-359; TRANS-RXN-367
Predicted SEED Role
"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A1SBK0 at UniProt or InterPro
Protein Sequence (1046 amino acids)
>Sama_3554 AcrB/AcrD/AcrF family protein (RefSeq) (Shewanella amazonensis SB2B) MARFFIDRPIFAWVIAILVMLAGVLAIKSLPVSQYPNIAPPTVVISANYPGASAKTLEDS VTQVIEQRMTGLDNLRYINSTSDGFGNAEITLTFNAEADADIAQVQVQNKLQSAMPLLPQ EVQAQGVRVNKSTSGFLMVLGFVSEDGSLTKNDIADYVGSNVQDPLSRVAGVGEITLFGA PYAMRIWLDPLKLTQYSLTTQDVIAAIREQNAQVSAGQLGGAPAVLGQELNATVSAQSRL ETPEQFRKIILKSDTNGAKVFLGDVARVELGAESYVVESFYNGRPAAGLAISLATGANAL DTATRVREKIAEMKPFFPAGLDVVYPYDTTPFVKQSIEGVVHTLLEAVVLVFVIMYLFLQ NFRATLIPTIAVPVVLLGTFAILSAFGFSINTLTMFAMVLAIGLLVDDAIVVVENVERVM QEEGLGPVEATRKSMDQITGALVGIGLTLSAVFVPMAFMSGSTGVIYRQFSITIVSAMAL SVLVALILTPALCATMLKPLKPGHSHGQHGFFGWFNRSFEKMTSRYEGSVAAILKRSARV MAIYLALVVATGWIFMRMPTAFLPDEDQGILFAQVILPTNSSQESTVKVLEKMDKFFRED EKDNVNSVFSVAGFSFAGMGQNMGIAFVGMKDWAVREGEGQDVFSLQGRAMMAFSQIKEA FVFAFAPPAVIELGTANGFDFYLQDKNGQGHAKLLEARNQLLGMAAQNPNLVGVRPNGQE DAPMYQLDIDHAKLRALGVDISSVNSAIATAWGGSYVNDFIDRGRVKKVYVQGDAEYRMQ PEDLNTWYVRNANGEMVPFSAFATGRWDFGSPRLERFNGLPAMNIQGATAPGYSTGDAMK DIEKIVEQLPPGFGVEWNGLSYEERLSGNQAPALYALSILVVFLVLAALYESWSVPFAVV LVVPLGIIGALLAMNGRGLSNDVFFQVGLLTTVGLATKNAILIVEFAKEYYEKGAGLIEA TLHAVRVRLRPILMTSLAFGLGVVPLAISTGVGSGAQNAIGTGVLGGMMSSTFLGIFFVP LFFVIVERIFSKREKQAQTGAGAGKH