Protein Info for Sama_3475 in Shewanella amazonensis SB2B
Annotation: CzcA family heavy metal efflux protein (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 58% identical to CUSA_ECOLI: Cation efflux system protein CusA (cusA) from Escherichia coli (strain K12)
KEGG orthology group: K07787, Cu(I)/Ag(I) efflux system membrane protein CusA (inferred from 64% identity to alv:Alvin_1523)MetaCyc: 58% identical to copper/silver export system RND permease (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-90; TRANS-RXN0-280
Predicted SEED Role
"Cobalt-zinc-cadmium resistance protein CzcA; Cation efflux system protein CusA"
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A1SBC1 at UniProt or InterPro
Protein Sequence (1048 amino acids)
>Sama_3475 CzcA family heavy metal efflux protein (RefSeq) (Shewanella amazonensis SB2B) MLDYIIKSSIRQRFMVLVVALMVTLWGITELKRTPLDALPDLSDVQVIIKTSFPGQAPQL VEEQVTYPLSTAMLAVPGAKTVRGFSMFGDSYVYVIFEDGTDIYWARSRVLEYLSQVRTR LPAGVEPSLGPDASGVGWVYEYALVDRSGNLDLSQLKSLQDWYLKLELQSVEGVSEVATV GGMEQTYQIVLEPDKLAIYKLDIATIKAAITRANSEAGGSVVEMAEAEYMVRAKGYRQTL DDFREIPLGITTGAGTPLLLKDVATIRKGPASRRGIAELDGEGEVVGGIIVMRYGENALA TIDAVKAKLDELKAGLPDGVEIVPTYDRSQLIQNSVDNLLTKVVEEMLVVGLVCLLFLLH ARSTLVAVFTLPLSILIAFIVMNHMGINANIMSLGGIAIAIGAVVDGAIVMIENLHKHLE HFKRQHEREPSTREHWEVVTQASLEVGPALFFSLLIITLSFIPVFALEAQEGRLFAPLAY TKTFAMAAASVLAITLVPVLMGYFIRGKIPSEEANPISRFLIALYKPALNKVLAFPKLTL LVALLALASVVYPASRMGSEFMPELEEGDLLYMPTALPGISAGKAAEILQQTDRLIKTVP EVARVFGKIGRAETATDPAPLTMLETTIMLKPRQEWREGINLNDIVDELQRTVKVPGLTN AWVQPIKTRIDMLSTGIKTPVGIKITGANVDELQQIGANVEAILAALPNTRSAYAERAGG GRYIDIAPKLDVASRYGMTLNDIQDVVRYAIGGMNVGESVQGAERYPINLRYPRELRDSL EKLRALPVITKSGHYLPLGNLADIQISDGPPMLKSENGRLISWVFVDIQGTSIGEYIQTA KSALEAELNLPPRYSYSFAGQYEYMQRVDAKLKQVVPLAIGIIFILLMLTFGSGKQASMI MLSLPFALVGSTWLLYLLDYNLSVAVAVGMIALAGVAAEFGVVMLVYLNNAIKDRDALGH YHNESDLKAALVEGAVMRIRPKAMTVATIFFGLLPIMWGAGSGNEVMQKIAAPMVGGMVT APLLSLFVLPALYLLVYRKRFAAKGDEQ