Protein Info for Sama_3353 in Shewanella amazonensis SB2B

Annotation: hypothetical protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 281 PF00590: TP_methylase" amino acids 7 to 206 (200 residues), 117.5 bits, see alignment E=8.1e-38 TIGR00096: 16S rRNA (cytidine(1402)-2'-O)-methyltransferase" amino acids 7 to 277 (271 residues), 329.3 bits, see alignment E=9.9e-103 PF23016: RsmI_C" amino acids 235 to 278 (44 residues), 50.8 bits, see alignment 1.2e-17

Best Hits

Swiss-Prot: 63% identical to RSMI_VIBCH: Ribosomal RNA small subunit methyltransferase I (rsmI) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K07056, (no description) (inferred from 100% identity to saz:Sama_3353)

MetaCyc: 64% identical to 16S rRNA 2'-O-ribose C1402 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11637 [EC: 2.1.1.198]

Predicted SEED Role

"rRNA small subunit methyltransferase I" in subsystem Heat shock dnaK gene cluster extended

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.198

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A1SAZ9 at UniProt or InterPro

Protein Sequence (281 amino acids)

>Sama_3353 hypothetical protein (RefSeq) (Shewanella amazonensis SB2B)
MSLPPALYIVPTPIGNLGDMSPRAVEILQSVSLIACEDTRHSGRLFSHFGISTRTTSVHD
HNERARAQWIIEKLSQGDAVALVSDAGTPLISDPGYHLVSQVREAGFAVIPLPGPCAAIT
ALSASGLPSDRFSFEGFLPAKDKGRNDKLTALREDPRTLIFYESPHRILQTLEAIVAVLG
GERPMVMARELTKTFETFLSGTAAEVLEKVKSDDNQQKGEMVLMCHGFVAEETEEAIPAK
AMETLALLATELPLKKAAAMAAQIHDLKKNALYKAGLDAGL